Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged

• Cat.No.: MAP2K1-2723HF
• Product Overview: Full-length human MAP2K1 [1-393(end) amino acids of accession number NP_002746.1] was co- expressed as N-terminal GST-fusion protein (71 kDa) with human His-tagged RAF1 [306-648(end) amino acids and Y340D and Y341D of accession number NP_002871.1] using baculovirus expression system. GST-MAP2K1 was purified by using glutathione sepharose chromatography and Ni-NTA affinity chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-393(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
• Purity: 90%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase kinase 1
• Official Symbol: MAP2K1
• Synonyms: MAP2K1;CFC3;MAPKK1;MEK1;MKK1;PRKMK1
• Protein Refseq: NP_002746.1
• UniProt ID: Q02750