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Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged

Cat.No. : MAP2K1-2723HF
Product Overview : Full-length human MAP2K1 [1-393(end) amino acids of accession number NP_002746.1] was co- expressed as N-terminal GST-fusion protein (71 kDa) with human His-tagged RAF1 [306-648(end) amino acids and Y340D and Y341D of accession number NP_002871.1] using baculovirus expression system. GST-MAP2K1 was purified by using glutathione sepharose chromatography and Ni-NTA affinity chromatography.
  • Specification
  • Gene Information
  • Related Products
Source : Insect (sf21)
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
Protein length : 1-393(end)
Purity : 90%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
Gene Name : mitogen-activated protein kinase kinase 1
Official Symbol : MAP2K1
Protein Refseq : NP_002746.1
UniProt ID : Q02750

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.



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