Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged

• Cat.No.: MAP2K1-3929HF
• Product Overview: Full-length human MAP2K1 [1-393(end) amino acids and P124L of accession number NP_002746.1] was co-expressed as N-terminal GST-fusion protein (70 kDa) with human His-tagged RAF1 [306-648 amino acids and Y340D and Y341D of accession number NP_002871.1] using baculovirus expression system. GST-MAP2K1[P124L] was purified by using glutathione sepharose chromatography and Ni-NTA affinity chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
• Protein length: 1-393(end)
• Purity: 97%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase kinase 1
• Official Symbol: MAP2K1
• Synonyms: MAP2K1;CFC3;MAPKK1;MEK1;MKK1;PRKMK1
• Protein Refseq: NP_002746.1
• UniProt ID: Q02750

Reviews

08/01/2020

The unmatched performance of the MAP2K1 protein in both ELISA assays and protein electron microscopy structure analysis showcases its versatility and reliability in various experimental settings.

05/18/2016

The MAP2K1 protein finds extensive utility in protein electron microscopy structure analysis.

04/11/2016

With its exceptional specificity and sensitivity, it enables precise and reliable detection of target molecules, making it a valuable tool in the field of diagnostic and research applications.

Q&As

Are there ongoing clinical trials targeting MAP2K1?

Yes, there are clinical trials exploring the efficacy and safety of MAP2K1 inhibitors in various cancers and other diseases.

Are there diagnostic tests specifically targeting MAP2K1 in clinical practice?

Some diagnostic tests may analyze MAP2K1 mutations, especially in the context of cancer, to inform treatment decisions.

What diagnostic applications does MAP2K1 have in cancer detection?

MAP2K1 mutations can serve as biomarkers in cancer diagnostics, aiding in the identification and classification of tumors.

How does MAP2K1 contribute to drug resistance in cancer treatment?

MAP2K1 mutations can be associated with resistance to certain cancer therapies, making it important to understand its role in treatment response.

Are there targeted therapies based on MAP2K1 in cancer treatment?

Yes, MAP2K1 inhibitors are being explored as targeted therapies in cancer treatment, aiming to block the abnormal signaling associated with MAP2K1 mutations.