ACOT8
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Official Full Name
acyl-CoA thioesterase 8
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Overview
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. -
Synonyms
ACOT8; acyl-CoA thioesterase 8; peroxisomal acyl CoA thioesterase , peroxisomal acyl CoA thioesterase 1 , PTE1; acyl-coenzyme A thioesterase 8; choloyl CoA hydrolase; hACTE III; hTE; PTE 2; ACTEIII; ACOT8_HUMAN; acyl CoA thioesterase 8; Choloyl coenzyme A thioesterase; Choloyl-coenzyme A thioesterase; hACTE-III; hACTEIII; HIV Nef associated acyl CoA thioesterase; HIV-Nef-associated acyl-CoA thioesterase; HNAACTE; Long chain fatty acyl CoA hydrolase; Palmitoyl CoA hydrolase; Peroxisomal acyl CoA thioesterase 1; Peroxisomal acyl coenzyme A thioester hydrolase 1; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long chain acyl CoA thioesterase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE 1; PTE-1; PTE-2; PTE1; PTE2; Thioesterase II; thioesterase III; OTTHUMP00000031675; OTTHUMP00000214962; choloyl-CoA hydrolase; palmitoyl-CoA hydrolase; long-chain fatty-acyl-CoA hydrolase; peroxisomal acyl-CoA thioesterase 1; HIV;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Human
- Mouse
- Rhesus Macaque
- Zebrafish
- E.coli
- E.Coli or Yeast
- HEK293
- In Vitro Cell Free System
- Mammalian Cell
- Wheat Germ
- GST
- His
- His (Fc)
- Avi
- N/A
Species | Cat.# | Product name | Source (Host) | Tag | Protein Length | Price |
---|---|---|---|---|---|---|
Human | ACOT8-3141H | Recombinant Human ACOT8, His-tagged | E.Coli or Yeast | His | 319 | |
Human | ACOT8-7604H | Recombinant Human ACOT8, His-tagged | E.coli | N/A | 1-319aa | |
Human | ACOT8-170H | Recombinant Human ACOT8 Protein, GST-Tagged | Wheat Germ | GST | ||
Human | ACOT8-9086HCL | Recombinant Human ACOT8 293 Cell Lysate | HEK293 | N/A | ||
Human | ACOT8-906H | Recombinant Human ACOT8 Protein, His-tagged | E.coli | His | ||
Human | ACOT8-793HF | Recombinant Full Length Human ACOT8 Protein, GST-tagged | In Vitro Cell Free System | GST | 319 amino acids | |
Mouse | Acot8-3140M | Recombinant Mouse Acot8, His-tagged | E.Coli or Yeast | His | 320 | |
Mouse | ACOT8-1206M | Recombinant Mouse ACOT8 Protein | Mammalian Cell | His | ||
Mouse | ACOT8-259M-B | Recombinant EPCAM-coupled magnetic beads | HEK293 | |||
Mouse | ACOT8-259M | Recombinant Mouse ACOT8 Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi | ||
Rhesus Macaque | ACOT8-213R | Recombinant Rhesus monkey ACOT8 Protein, His-tagged | Mammalian Cell | His | ||
Rhesus Macaque | ACOT8-41R | Recombinant Rhesus Macaque ACOT8 Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi | ||
Rhesus Macaque | ACOT8-41R-B | Recombinant Rhesus Macaque ACOT8 Protein Pre-coupled Magnetic Beads | HEK293 | |||
Zebrafish | ACOT8-1581Z | Recombinant Zebrafish ACOT8 | Mammalian Cell | His |
- Involved Pathway
- Protein Function
- Interacting Protein
ACOT8 involved in several pathways and played different roles in them. We selected most pathways ACOT8 participated on our site, such as Beta-oxidation of pristanoyl-CoA, Beta-oxidation of very long chain fatty acids, Bile acid and bile salt metabolism, which may be useful for your reference. Also, other proteins which involved in the same pathway with ACOT8 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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Beta-oxidation of pristanoyl-CoA | ACOT8;SCP2B;CRAT |
Beta-oxidation of very long chain fatty acids | ACOT8;CRAT |
Bile acid and bile salt metabolism | SLC27A2A;CYP46A1;CYP8B2;NCOA2;SLCO1B1;Alb;AKR1C6;CYP7A1;BRSK2 |
Fatty Acyl-CoA Biosynthesis | ACOT11B;ACOT11;ACOT7;ACOT13;ACOT10;ACOT11A;ACOT9;THEM5;ACOT8 |
Fatty acid, triacylglycerol, and ketone body metabolism | ACSF2;CPT2;ACOT11;CTGF;DGAT1B;ACOT11A;AGPAT2;MMAA;PLIN2 |
Metabolic pathways | NT5C1A;CYP4A10;NAPRT;PLA2G3;PLCD1A;ALOX8;CKMT1;GAD2;PIGB |
Metabolism | SLC25A15;ACOT9.2;OAZ2;DPEP1;CHST14;CBLC-1;ABCA1;MED31;GC |
Metabolism of lipids and lipoproteins | ALOX5B.3;CYP8B2;PLBD1;SLCO1B1;ACOT6;NEU3;FIG4;ACOT10;BCHE |
ACOT8 has several biochemical functions, for example, acyl-CoA hydrolase activity, carboxylic ester hydrolase activity, choloyl-CoA hydrolase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by ACOT8 itself. We selected most functions ACOT8 had, and list some proteins which have the same functions with ACOT8. You can find most of the proteins on our site.
Function | Related Protein |
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acyl-CoA hydrolase activity | ACOT8;ACOT2;ACOT5;ACSBG2;ACOT10;ACOT1;ACOT11 |
carboxylic ester hydrolase activity | CES2;ABHD2;ACOT12;ABHD2A;PNPLA7;Cel;ACOT7;ACOT5;AADACL4 |
choloyl-CoA hydrolase activity | |
medium-chain acyl-CoA hydrolase activity | ACOT8;BAAT |
palmitoyl-CoA hydrolase activity | THEM4;THEM5;ACOT2;ACOT5;ACOT8;ACOT7;BAAT;PPT1;ACOT1 |
protein binding | SYTL1;PRICKLE1A;GPAA1;ADRA2C;C12orf50;REXO1L1;SNRNP40;GNPDA2;ZNF785 |
receptor binding | FGF1B;IDH1;ALCAM;RCHY1;PTPRD;MIF;PLAT;NTF3;HAMP |
ACOT8 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ACOT8 here. Most of them are supplied by our site. Hope this information will be useful for your research of ACOT8.
nef; PEX5; REL; 15-deoxy-delta(12,14; midostaurin; MYC; US3; Nedd1; P
- Q&As
- Reviews
Q&As (13)
Ask a questionACOT7 is expressed in a variety of tissues, including adipose tissue, liver, and skeletal muscle, where it plays important roles in regulating lipid metabolism and energy production. In adipose tissue, ACOT7 helps to control adiposity and improve glucose tolerance, while in liver it helps to reduce the accumulation of toxic lipids that are associated with NAFLD. In skeletal muscle, ACOT7 may help to regulate the production of ATP and the breakdown of glycogen, which are important for exercise performance.
Yes, ACOT7 protein has been proposed as a drug target for treating metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease. However, more research is needed to fully understand the mechanisms of action and develop safe and effective ACOT7-targeted therapies.
The regulation of ACOT7 in cells is complex and not completely understood. Several studies have suggested that ACOT7 expression and activity may be regulated by factors such as fatty acid availability, insulin signaling, and post-translational modifications such as phosphorylation and acetylation. However, the precise mechanisms by which these factors regulate ACOT7 are still being investigated.
Several experimental techniques are used to study ACOT7, including genetic manipulation in cells and animal models, biochemical assays, and structural studies. For example, genetic manipulation techniques such as RNA interference and CRISPR/Cas9 can be used to study the effects of ACOT7 knockdown or overexpression in cells. Biochemical assays such as thin-layer chromatography and mass spectrometry are used to measure the activity of ACOT7 and its substrate specificity. Structural studies such as X-ray crystallography and nuclear magnetic resonance spectroscopy are used to determine the 3D structure of ACOT7 and its interaction with substrates and inhibitors.
ACOT7 protein has potential clinical applications in the treatment of metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease. It may also have a potential role in improving exercise endurance and muscle health.
The dysregulation of ACOT7 has been implicated in several metabolic disorders, suggesting that it could be a potential target for drug development. However, several challenges must be overcome in developing ACOT7-targeted therapies, including the need to develop inhibitors that are selective for ACOT7 and do not inhibit other acyl-CoA thioesterases with different functions. Furthermore, given the crucial role of acyl-CoA esters in lipid metabolism and energy production, careful consideration must be given to potential side effects of ACOT7 inhibition.
ACOT7 protein may play a role in regulating energy production and glucose metabolism in skeletal muscle, which can affect exercise performance. Overexpression of ACOT7 in skeletal muscle has been shown to improve exercise endurance and increase glucose uptake in animal models. However, more research is needed to fully understand the role of ACOT7 in exercise physiology and its potential use in improving exercise performance.
Dysregulation of ACOT7 has been implicated in several metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Studies in animal models have shown that overexpression of ACOT7 in adipose tissue can lead to improved glucose tolerance and reduced adiposity, suggesting a potential therapeutic role for ACOT7 in metabolic disorders.
ACOT7 plays an important role in lipid metabolism by catalyzing the hydrolysis of acyl-CoA esters, which are important intermediates in lipid metabolism. By breaking down acyl-CoA esters, ACOT7 helps regulate the levels of these intermediates and prevent excessive accumulation of lipids that can lead to metabolic disorders such as NAFLD. Additionally, studies have suggested that ACOT7 may play a role in regulating lipid oxidation and energy production in skeletal muscle.
Several natural compounds have been identified that can inhibit ACOT7 activity, including flavonoids such as quercetin and orientin, and polyphenols such as resveratrol and curcumin. However, the specificity and potency of these compounds and their potential therapeutic use in metabolic disorders requires further study.
One potential technique for delivering ACOT7 protein to target tissues is through the use of gene therapy, in which a gene encoding for ACOT7 is introduced into target cells using viral vectors. Another potential approach is the use of cell-based therapies using stem cells or other cell types that overexpress ACOT7. However, both of these approaches require further research to assess their safety and efficacy.
One of the main challenges in targeting ACOT7 for therapeutic purposes is to achieve specific inhibition of ACOT7 without affecting other proteins that share similar functions in lipid metabolism. Additionally, there may be potential side effects associated with modulating ACOT7 activity, and the long-term safety and efficacy of ACOT7-targeted therapies needs to be carefully evaluated.
The role of ACOT7 in exercise physiology is not fully understood, but studies have suggested that it may play a role in regulating energy production and glucose metabolism in skeletal muscle. In animal models, overexpression of ACOT7 in skeletal muscle has been shown to improve exercise endurance and increase glucose uptake. Additionally, levels of ACOT7 in skeletal muscle have been shown to increase in response to exercise, suggesting a potential role in adaptation to physical activity.
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