Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953 Learn More

LIG4

Official Full Name

ligase IV, DNA, ATP-dependent
Overview

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Synonyms

LIG4; ligase IV, DNA, ATP-dependent; DNA ligase 4; sealase; DNA joinase; DNA ligase IV; DNA repair enzyme; polynucleotide ligase; polydeoxyribonucleotide synthase [ATP] 4;

Type

Recombinant Proteins
Cell & Tissue Lysates
Protein Pre-coupled Magnetic Beads

Species

Chicken
Human
Mouse
Zebrafish

Source

HEK293
Mammalian Cell
Wheat Germ

Tag

GST
His
His (Fc)
Avi
N/A

Species	Cat.#	Product name	Source (Host)	Tag	Protein Length	Price
Human	LIG4-508H	Recombinant Human LIG4 protein, GST-tagged	Wheat Germ	GST	802 a.a. - 911 a.a.	Inquiry
Human	LIG4-4745HCL	Recombinant Human LIG4 293 Cell Lysate	HEK293	N/A		Inquiry
Human	LIG4-4746HCL	Recombinant Human LIG4 293 Cell Lysate	HEK293	N/A		Inquiry
Mouse	LIG4-9098M	Recombinant Mouse LIG4 Protein	Mammalian Cell	His		Inquiry
Mouse	LIG4-5079M	Recombinant Mouse LIG4 Protein, His (Fc)-Avi-tagged	HEK293	His (Fc)-Avi		Inquiry
Mouse	LIG4-5079M-B	Recombinant Mouse LIG4 Protein Pre-coupled Magnetic Beads	HEK293			Inquiry
Zebrafish	LIG4-5960Z	Recombinant Zebrafish LIG4	Mammalian Cell	His		Inquiry
Chicken	LIG4-2513C	Recombinant Chicken LIG4	Mammalian Cell	His		Inquiry

Involved Pathway
Protein Function
Interacting Protein
LIG4 Related Articles

LIG4 involved in several pathways and played different roles in them. We selected most pathways LIG4 participated on our site, such as Non-homologous end-joining, which may be useful for your reference. Also, other proteins which involved in the same pathway with LIG4 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name	Pathway Related Protein
Non-homologous end-joining	LIG4;DNTT;DCLRE1C;POLM;NHEJ1;FEN1;XRCC5;PRKDC;XRCC6

LIG4 has several biochemical functions, for example, ATP binding, DNA binding, DNA ligase (ATP) activity. Some of the functions are cooperated with other proteins, some of the functions could acted by LIG4 itself. We selected most functions LIG4 had, and list some proteins which have the same functions with LIG4. You can find most of the proteins on our site.

Function	Related Protein
ATP binding	ACSL4;STYK1;ABCB3L1;FAM20C;ABCC2;SCN8A;ABCG2;XRCC5;POMK
DNA binding	HIST1H2BM;RFXAP;TBPL1;THAP4;TNP1;BRD4;RORCB;HOXC5A;ETV5A
DNA ligase (ATP) activity	LIG4;LIG3;LIG1
DNA ligase activity	LIG3;LIG1;LIG4
ligase activity	UBE2NB;BIRC2;RAD18;BRCA1;SH3RF2;CMTM2A;TRIM58;HECTD1;CPS1
metal ion binding	GFI1B;ATF7B;PPM1DB;CMAH;ZNF192;ATF7;ACACB;BGLAP2;SALL1
protein C-terminus binding	ZBTB16;TBL1X;SIAH1;TAE1;OPRL1;SHANK2;CDC37;PDZD11;JAK2
protein binding	AKR1B10;WDYHV1;CHRNB2;Tnfrsf23;CALCOCO2;ADAMTS4;MTERF;BCKDK;DYNLT3

LIG4 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with LIG4 here. Most of them are supplied by our site. Hope this information will be useful for your research of LIG4.

XRCC4; APLF; NHEJ1; PNKP; TSFM; GZMK; ZSCAN18; PHF10; XRCC6

Cordoba-Canero, D; Roldan-Arjona, T; et al. Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1. PLANT JOURNAL 68:693-702(2011).

Paddock, MN; Bauman, AT; et al. Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair. DNA REPAIR 10:338-343(2011).