Active Recombinant Human BTK protein, N-His-tagged

• Cat.No.: BTK-398H
• Product Overview: Active Recombinant Human BTK protein(NP_000052.1) (Met 1-Ser 659) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-659 aa
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4.
• Bio-activity: 1. The specific activity was determined to be 115 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate. 2. Measured by its ability to bind human BLNK in a functional ELISA.
• Molecular Mass: The recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Official Symbol: BTK
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; AGMX1, IMD1; tyrosine-protein kinase BTK; ATK; PSCTK1; XLA; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase; AT; BPK; IMD1; AGMX1; MGC126261; MGC126262
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187