Recombinant Full Length Human BTK Protein, C-Flag-tagged

• Cat.No.: BTK-985HFL
• Product Overview: Recombinant Full Length Human BTK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 76.1 kDa
• AA Sequence: MAAVILESIFLKRSQQKKKTSPLNF KKRLFLLTVHKLSYYEYDFERGRRG SKKGSIDVEKITCVETVVPE KNPPPERQIPRRGEESSEMEQISII ERFPYPFQVVYDEGPLYVFSPTEEL RKRWIHQLKNVIRYNSDLVQ KYHPCFWIDGQYLCCSQTAKNAMGC QILENRNGSLKPGSSHRKTKKPLPP TPEEDQILKKPLPPEPAAAP VSTSELKKVVALYDYMPMNANDLQL RKGDEYFILEESNLPWWRARDKNGQ EGYIPSNYVTEAEDSIEMYE WYSKHMTRSQAEQLLKQEGKEGGFI VRDSSKAGKYTVSVFAKSTGDPQGV IRHYVVCSTPQSQYYLAEKH LFSTIPELINYHQHNSAGLISRLKY PVSQQNKNAPSTAGLGYGSWEIDPK DLTFLKELGTGQFGVVKYGK WRGQYDVAIKMIKEGSMSEDEFIEE AKVMMNLSHEKLVQLYGVCTKQRPI FIITEYMANGCLLNYLREMR HRFQTQQLLEMCKDVCEAMEYLESK QFLHRDLAARNCLVNDQGVVKVSDF GLSRYVLDDEYTSSVGSKFP VRWSPPEVLMYSKFSSKSDIWAFGV LMWEIYSLGKMPYERFTNSETAEHI AQGLRLYRPHLASEKVYTIM
  YSCWHEKADERPTFKILLSNILDVM DEESTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency

Gene Information

• Gene Name: BTK Bruton tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BTK
• Synonyms: AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
• Gene ID: 695
• mRNA Refseq: NM_000061.3
• Protein Refseq: NP_000052.1
• MIM: 300300
• UniProt ID: Q06187