Active Recombinant Human MAP4K1(1-346) Protein, C-His,N-GST-tagged

• Cat.No.: MAP4K1-103H
• Product Overview: N-terminal GST tagged and C-terminal 6His-tagged, recombinant, human HPK1, amino acids 1- 346 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: HPK1 is a Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 1-346 aa
• Form: 0.41mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1864U/mg, where one unit of HPK1, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKELM DVVDPDIFNR 241 DPRDHYDLLQ RLGGGTYGEV FKARDKVSGD LVALKMVKME PDDDVSTLQK EILILKTCRH 301 ANIVAYHGSY LWLQKLWICM EFCGAGSLQD IYQVTGSLSE LQISYVCREV LQGLAYLHSQ 361 KKIHRDIKGA NILINDAGEV RLADFGISAQ IGATLARRLS FIGTPYWMAP EVAAVALKGG 421 YNELCDIWSL GITAIELAEL QPPLFDVHPL RVLFLMTKSG YQPPRLKEKG KWSAAFHNFI 481 KVTLTKSPKK RPSATKMLSH QLVSQPGLNR GLILDLLDKL KNPGKGPSIG DIEDEEPELP 541 PAIPRRIRST HRSSSLGIPD ADCCRRHMEF RKLRGGPGPG HHHHHH
• Purity: Purity 81% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP4K1 mitogen-activated protein kinase kinase kinase kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MAP4K1
• Synonyms: MAP4K1; mitogen-activated protein kinase kinase kinase kinase 1; HPK1
• Gene ID: 11184
• mRNA Refseq: NM_007181.6
• Protein Refseq: NP_009112.1
• MIM: 601983
• UniProt ID: Q92918