Recombinant Human MAP4K1 Protein, GST-tagged

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Recombinant Human MAP4K1 Protein, GST-tagged

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Cat.No. : MAP4K1-79H
Product Overview : Recombinant full-length human HPK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description : HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. HPK1 is also known as mitogen-activated protein kinase kinase kinase kinase 1(MAP4K1). HPK1 is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. HPK1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76.
Source : Insect cells
Species : Human
Tag : GST
Molecular Mass : ~121 kDa
Purity : > 70%
Applications : Kinase Assay, WB
Stability : One year at -70 centigrade from date of shipment.
Storage : Store at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.05 μg/μL
Storage Buffer : 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol
Specific Activity :

The specific activity of HPK1 was determined to be 45.9 nmol/min/mg as per activity assay protocol.

Purity :

The purity of HPK1 was determined to be >70% by densitometry.

Gene Name : MAP4K1 mitogen-activated protein kinase kinase kinase kinase 1 [ Homo sapiens (human) ]
Official Symbol : MAP4K1
Synonyms : HPK1; mitogen-activated protein kinase kinase kinase kinase 1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; hematopoietic progenitor kinase 1; EC 2.7.11.1
Gene ID : 11184
mRNA Refseq : NM_007181
Protein Refseq : NP_009112
MIM : 601983
UniProt ID : Q92918

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