What is RCHY1 Protein

What is RCHY1 Protein?

The RCHY1 (the RING finger and CHY zinc finger domain-containing protein 1), also known as Pirh-2, is a multifaceted protein encoded by the RCHY1 gene. This gene is situated on human chromosome 4q24, and the RCHY1 protein it encodes plays pivotal roles in various cellular processes. Structurally characterized by a Ring Finger domain and a CHY Zinc Finger domain, RCHY1 orchestrates intricate cellular processes. This protein's intricate structure lays the foundation for its multifaceted functions.

Figure 1. Secondary sequence organization of the CHY-zinc-finger/RING-finger domain. (Jung, Y.S., et al. 2012)

The Function of RCHY1 Protein

1. Ubiquitin Ligase Activity

At the molecular level, RCHY1 operates as an E3 ubiquitin ligase, facilitating the transfer of ubiquitin molecules to specific target proteins. This ubiquitination marks proteins for degradation by the proteasome, a process vital for cellular homeostasis. An exemplary instance of this function is its interaction with the tumor suppressor protein p53.

1. p53 Regulation

RCHY1 engages in a complex interplay with p53, a key guardian of genomic integrity. By promoting ubiquitination and subsequent degradation of p53, RCHY1 regulates cellular responses to stress, DNA damage, and other signaling cues. This interaction underscores the protein's role in preventing the accumulation of excessive p53, finely tuning cellular stress responses.

1. Cell Cycle Control

The regulatory influence of RCHY1 extends to the cell cycle, where it modulates the stability of proteins governing cell division and proliferation. Through precise orchestration of these events, RCHY1 ensures normal cell growth and prevents uncontrolled proliferation, contributing to the maintenance of cellular integrity.

RCHY1-Related Diseases

1. Cancer

The intricate involvement of RCHY1 in the regulation of p53 positions it at the forefront of cancer research. Dysregulation or mutations in the RCHY1 gene have been associated with various cancers, emphasizing its potential as a therapeutic target. By modulating RCHY1 activity, researchers aim to restore p53 function, triggering apoptosis and inhibiting tumor progression.

1. Neurological Disorders

Emerging research suggests a connection between RCHY1 and neurological disorders. As a participant in cellular processes crucial for neuronal function, RCHY1's implications in neurodegenerative diseases prompt further investigation. Unraveling its role in neurological contexts may unveil novel therapeutic strategies.

RCHY1 Related Signaling Pathways

1. p53 Signaling Pathway

The nexus between RCHY1 and the p53 signaling pathway is paramount. By influencing p53 levels, RCHY1 plays a pivotal role in modulating cellular responses to stressors and maintaining genomic stability. This intricate signaling cascade highlights RCHY1 as a key orchestrator in the cellular stress response.

1. NF-κB Signaling Pathway

Beyond p53, RCHY1 intersects with the NF-κB signaling pathway, known for its involvement in inflammation, immunity, and cell survival. While the precise mechanisms remain under scrutiny, RCHY1's interaction with NF-κB components offers insights into immune responses and inflammatory processes.

Applications of RCHY1 in Biomedical Research

1. Cancer Therapeutics

Exploiting RCHY1 as a potential target opens avenues for cancer therapeutics. The protein's regulation of p53 presents an innovative approach to cancer treatment. Modulating RCHY1 activity may offer a means to restore p53 function in cancer cells, inducing apoptosis and inhibiting tumorigenesis.

1. Neurological Disorder Research

Understanding RCHY1's implications in neurological disorders provides a springboard for research and therapeutic development. As RCHY1 influences processes crucial for neuronal function, exploring its role in neurodegenerative diseases may pave the way for novel interventions.

1. Drug Development

RCHY1's involvement in diverse cellular processes positions it as an attractive target for drug development. Small molecules or compounds that modulate RCHY1 activity hold promise for influencing cellular regulation beyond cancer and neurological disorders. The potential applications of such compounds in various disease contexts make RCHY1 a focal point in drug discovery efforts.

From its ubiquitin ligase activity to its role in regulating pivotal signaling pathways, RCHY1's impact spans cellular regulation, disease etiology, and biomedical applications. As researchers unravel the complexities of RCHY1, the prospect of therapeutic interventions and drug development in cancer, neurological disorders, and beyond beckons on the horizon of scientific exploration.

Recommended Products for RCHY1 Protein

Cat.#	Species	Product name	Source (Host)	Tag
RCHY1-233H	Human	Recombinant Human ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase, His-tagged	E.coli	His
RCHY1-4559H	Human	Recombinant Human RCHY1 Protein, Myc/DDK-tagged, C13 and N15-labeled	HEK293T	Myc/DDK
RCHY1-4646H	Human	Recombinant Human RCHY1 protein, GST-tagged	E.coli	GST
RCHY1-234H	Human	Recombinant Human RCHY1 Protein, Myc/DDK-tagged	HEK293T	Myc/DDK
RCHY1-14034M	Mouse	Recombinant Mouse RCHY1 Protein	Mammalian Cell	His
Rchy1-5437M	Mouse	Recombinant Mouse Rchy1 Protein, Myc/DDK-tagged	HEK293T	Myc/DDK
RCHY1-3833R	Rhesus Macaque	Recombinant Rhesus monkey RCHY1 Protein, His-tagged	Mammalian Cell	His
RCHY1-3650R	Rhesus Macaque	Recombinant Rhesus Macaque RCHY1 Protein, His (Fc)-Avi-tagged	HEK293	His (Fc)-Avi
RCHY1-11879Z	Zebrafish	Recombinant Zebrafish RCHY1	Mammalian Cell	His
RCHY1-3643C	Chicken	Recombinant Chicken RCHY1	Mammalian Cell	His

Reference

• Jung, Y.S., et al. Pirh2 RING-finger E3 ubiquitin ligase: its role in tumorigenesis and cancer therapy. FEBS Lett. 2012, 586(10): 1397-402.