Recombinant Human RCHY1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RCHY1-4559H
• Product Overview: RCHY1 MS Standard C13 and N15-labeled recombinant protein (NP_056251) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 30.1 kDa
• AA Sequence: MAATAREDGASGQERGQRGCEHYDR GCLLKAPCCDKLYTCRLCHDNNEDH QLDRFKVKEVQCINCEKIQHAQQTC EECSTLFGEYYCDICHLFDKDKKQY HCENCGICRIGPKEDFFHCLKCNLC LAMNLQGRHKCIENVSQQNCPICLE DIHTSRVVAHVLPCGHLLHRTCYEE MLKEGYRCPLCMHSALDMTRYWRQL DDEVAQTPMPSEYQNMTVDILCNDC NGRSTVQFHILGMKCKICESYNTAQ AGGRRISLDQQTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RCHY1 ring finger and CHY zinc finger domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: RCHY1
• Synonyms: RCHY1; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; ring finger and CHY zinc finger domain containing 1, zinc finger protein 363, ZNF363; RING finger and CHY zinc finger domain-containing protein 1; androgen receptor N terminal interacting protein; ARNIP; CHIMP; DKFZp586C1620; p53 induced protein with a RING H2 domain; PIRH2; PRO1996; RNF199; hPirh2; RING finger protein 199; zinc finger protein 363; p53-induced RING-H2 protein; E3 ubiquitin-protein ligase Pirh2; CH-rich interacting match with PLAG1; p53-induced protein with a RING-H2 domain; androgen-receptor N-terminal-interacting protein; PIRH2E; PIRH2F; ZNF363; hARNIP
• Gene ID: 25898
• mRNA Refseq: NM_015436
• Protein Refseq: NP_056251
• MIM: 607680
• UniProt ID: Q96PM5