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  • Official Full Name
  • Chloramphenicol acetyltransferase
  • Background
  • Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme (EC 2.3.1.28) that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria. This enzyme covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, which prevents chloramphenicol from binding to ribosomes. A histidine residue, located in the C-terminal section of the enzyme, plays a central role in its catalytic mechanism. The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilised by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilisation is provided by an unusual interaction with a main-chain carbonyl oxygen.
  • Synonyms
  • E. coli Chloramphenicol acetyltransferase; E. coli CAT; Escherichia coli Chloramphenicol acetyltransferase; Escherichia coli CAT; CAT
Cat.#:CAT-0443HTag:GST
Source (Host):Wheat GermSpecies:Human
Product nameRecombinant Human CAT Protein, GST-Tagged
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Cat.#:CAT-2728HTag:MYC/DDK
Source (Host):HEK293Species:Human
Product nameRecombinant Human CAT Protein, MYC/DDK-tagged
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Cat.#:CAT-654HTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human CAT Protein, His-tagged
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Cat.#:Cat-658MTag:His
Source (Host):E. coliSpecies:Mouse
Product nameRecombinant Mouse Cat Protein, His-tagged
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Cat.#:Cat-653RTag:His
Source (Host):E. coliSpecies:Rat
Product nameRecombinant Rat Cat Protein, His-tagged
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Cat.#:CAT-1646HTag:N/A
Source (Host):Human erythrocytesSpecies:Human
Product nameRecombinant Human Catalase
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Cat.#:CAT-2514HTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human Catalase, His-tagged
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Cat.#:CAT-167HTag:His
Source (Host):Mammalian CellsSpecies:Human
Product nameRecombinant Human CAT
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Cat.#:CAT-176HTag:
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human CAT Protein
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Cat.#:CAT-2500HTag:His
Source (Host):Baculovirus-Insect CellsSpecies:Human
Product nameRecombinant Human CAT, His tagged
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Cat.#:Cat-25MTag:His
Source (Host):E. coliSpecies:Mouse
Product nameRecombinant Mouse Cat protein, His-tagged
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Cat.#:Cat-26MTag:His
Source (Host):E. coliSpecies:Mouse
Product nameRecombinant Mouse Cat protein, His-tagged
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Cat.#:CAT-2765MTag:His
Source (Host):Mammalian CellsSpecies:Mouse
Product nameRecombinant Mouse CAT Protein
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Cat.#:CAT-1150RTag:His
Source (Host):Mammalian CellsSpecies:Rat
Product nameRecombinant Rat CAT Protein
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Cat.#:CAT-2878CTag:His
Source (Host):Mammalian CellsSpecies:Chicken
Product nameRecombinant Chicken CAT
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Cat.#:CAT-8553ZTag:His
Source (Host):Mammalian CellsSpecies:Zebrafish
Product nameRecombinant Zebrafish CAT
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Cat.#:CAT-1537STag:His
Source (Host):E. coli or YeastSpecies:Staphylococcus
Product nameRecombinant Staphylococcus aureus (strain: TP4) CAT protein, His-tagged
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Cat.#:CAT-1568STag:His
Source (Host):E. coli or YeastSpecies:Staphylococcus
Product nameRecombinant Staphylococcus aureus (strain: EP5) CAT protein, His-tagged
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Cat.#:CAT-21HTag:
Source (Host):Species:Human
Product nameNative Human Catalase Protein
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Cat.#:CAT-26409THTag:
Source (Host):Species:Human
Product nameNative Human CAT
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Cat.#:CAT-5276HTag:N/A
Source (Host):Human erythrocytesSpecies:Human
Product nameNative Human, Catalase
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Cat.#:CAT-75HTag:
Source (Host):Species:Human
Product nameNative Human Catalase
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Cat.#:CAT-101BTag:N/A
Source (Host):Bovine LiverSpecies:Bovine
Product nameNative Bovine CAT
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Cat.#:CAT-1187BTag:N/A
Source (Host):Bovine LiverSpecies:Bovine
Product nameNative Bovine Catalase
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Cat.#:CAT-1847BTag:N/A
Source (Host):Bovine LiverSpecies:Bovine
Product nameNative Bovine, Catalase
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Cat.#:CAT-515HCLTag:
Source (Host):Baculovirus-Insect CellsSpecies:Human
Product nameRecombinant Human CAT cell lysate
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Involved Pathway

CAT involved in several pathways and played different roles in them. We selected most pathways CAT participated on our site, such as Tryptophan metabolism, Glyoxylate and dicarboxylate metabolism, Carbon metabolism, which may be useful for your reference. Also, other proteins which involved in the same pathway with CAT were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name
Pathway Related Protein
Tryptophan metabolism
KMO; ANI_1_2390104; IL4I1; CYP1A; ALDH9A1; ALDH7A1; OGDH; TDO2; KYNU; ACAT1
Glyoxylate and dicarboxylate metabolism
PCCA; GRHPR; ACAT1; ACAT2; MCEE; GLULB; AMT; GLYCTK; GRHPRB; DHDPSL
Carbon metabolism
GOT2A; ME2; HAO2; MUT; SDHA; FH; MDH1; SDS; ENO1; DAK
FoxO signaling pathway
SMAD3; GABARAP; PIK3CA; IGF1; CCNB3; G6PCA.2; HOMER1B; TGFBR2; FOXO3A; SGK1
Peroxisome
PEX11B; PEX16; ECI2; PEX11A; PEX1; PEX3; DECR2; ACOX2; PEX2; SLC27A2
Amyotrophic lateral sclerosis (ALS)
DAXX; APAF1; GRIA1; CYCS; RAC1; BCL2L1; CASP1; NEFL; CASP9; PPP3CB

Protein Function

CAT has several biochemical functions, for example, NADP binding, aminoacylase activity, antioxidant activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CAT itself. We selected most functions CAT had, and list some proteins which have the same functions with CAT. You can find most of the proteins on our site.
Function
Related Protein
Function NADP binding
Related Protein H6PD; FMO2; FMO1; MTHFR; G6PD; SPR; FMO5; NNT; NOS3; HMGCRA
Function aminoacylase activity
Related Protein DARS; ASPA; ACY1; CAT; ACY3
Function antioxidant activity
Related Protein PRDX5; PRDX2; APOA4; PRDX6; FAM213AA; SEPW1; KDM3B; TP53INP1; SELS; HP
Function catalase activity
Related Protein CAT; GPX7; CYGB
Function enzyme binding
Related Protein KMT2E; PPP3CA; CAV1; ESR2; YWHAE; KAT8; BRCA1; ADORA2A; TSPAN17; KDM4C
Function heme binding
Related Protein CYP2B19; HBAD; AMBP; CYP2AA6; CYP8B3; HEBP1; HBB-BH1; HBE; CYP39A1; CYP4A12A
Function metal ion binding
Related Protein LNX2B; ZNHIT6; RHEBL1; IRF2BPL; ZNF280C; ZNF507; ZNF324; CYGB2; CA15C; RHOV
Function oxidoreductase activity, acting on peroxide as acceptor
Related Protein CAT; PARK7
Function protein homodimerization activity
Related Protein ERP29; CEBPE; TCF4; TIGIT; GCA; PON1; SLC51A; Il6ra; PDGFC; ADRA2C
Function receptor binding
Related Protein HSD17B4; SSFA2; HLA-G; NCR3; CDH5; EPB49; GNPAT; ACOT2; LEPROT; APOF

Interacting Protein

CAT has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CAT here. Most of them are supplied by our site. Hope this information will be useful for your research of CAT.
PTPN11; APP; APP; USP53; o68769_yerpe; p29991-pro_0000037946; KEAP1; proS; fcuA; leuB; 15-deoxy-delta(12,14; midostaurin; JUP; SMARCA4; ACTR3; GNAS

CAT Related Articles

Butz, E; Peichl, L; et al. Cone Bipolar Cells in the Retina of the Microbat Carollia perspicillata. JOURNAL OF COMPARATIVE NEUROLOGY 523:963-981(2015).
Cong, W; Liu, GH; et al. Toxoplasma gondii infection in cancer patients: Prevalence, risk factors, genotypes and association with clinical diagnosis. CANCER LETTERS 359:307-313(2015).