NFKBIA

  • Official Full Name

    nuclear factor of kappa light polypeptide gene enhancer in B-cell extracts inhibitor, alpha

  • Overview

    IκBα (Inhibitor of Nuclear Factor (NF)-κB α isoform) is a member of the IκB protein family with a MW of 40 kDa. IκBα is ubiquitously expressed among mammals. Located at IκBα's N-terminus is a signal response domain which contains serine residues that can be phosphorylated, while the C-terminus contains a PEST domain, a common feature among proteins with high turnover rates. As with all members of the IκB family, IκBα possesses ankyrin repeats approximately 33 amino acids in length, which mediate binding to the Rel homology region of NF-κB. The interaction of IκBα with NF-κB masks the nuclear localization sequence of NF-κB, preventing NF-κB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF-κB through the degradation of IκBα. These stimuli include the proinflammatory cytokines TNF-α and IL-1β, chemokines, PMA, growth factors, LPS, UV irradiation, and viral infection, as well as various chemical and physical stresses. The series of events leading to this liberation is well-defined. In response to stimulus, Ser32/36 of IκBα are phosphorylated, which provides a signal for IκBα E3 ligase, a protein complex composed of SKP-1, Cul-1, Roc1, and Fbw1. IκBα E3 ligase polyubiquitinates IκBα at lysine residues 21 and 22, and the polyubiquitinated IκBα is then targeted to the 26S proteasome for degradation. Liberated NF-κB is transported across the nuclear membrane, where it activates transcription by binding to the consensus sequence GGRNNYYCC, which is found in the promoter regions of a large number of genes including IL-6, VEGF, VCAM-1, ICAM-1, HIV long terminal repeat, and many others. The initial event which targets IκBα for degradation is phosphorylation of Ser32/36. This phosphorylation is catalyzed by a protein complex known as IKK (IκB kinase). IKK contains two kinase subunits designated IKKα (MW=85 kDa) and IKKβ (MW=87 kDa), and scaffold protein designated IKKγ/NEMO (MW=48 kDa). The kinetic properties of IKK are regulated by complex formation as well as by phosphorylation events catalyzed by upstream kinases including members of the MAPK cascade, the SAPK/JNK cascade and NIK. Through its regulation of NF-κB, IκBα controls immune and inflammatory responses, cell division, and apoptosis. Numerous disease states including arthritis, asthma, and inflammatory bowel disease are associated with loss of IκBα regulation. The importance of regulation of IκBα in cancer is underscored by the observation that multiple myelomas often possess polymorphisms at IκBα regulatory sites, and certain symptoms can be ameliorated using the proteasome inhibitor PS-341 which favors the sequestration of NF-κB in the cytoplasm.
  • Synonyms

    IKBA; MAD-3; NFKBI; I-kappa-B-alpha; IkappaBalpha; NF-kappa-B inhibitor alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Antibody
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • E. coli
  • E.coli
  • HEK293
  • HEK293T
  • Mammalian Cell
  • Rabbit
  • GST
  • His
  • His (Fc)
  • Avi
  • His|MBP
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human NFKBIA-4861H Recombinant Human NFKBIA, GST-tagged E.coli GST
Human NFKBIA-163H Recombinant Human NFKBIA protein, His/MBP-tagged E.coli His/MBP
Human NFKBIA-1463H Recombinant Human NFKBIA, GST-tagged E.coli GST
Human NFKBIA-90H Recombinant Human IKB-alpha, GST-tagged E.coli GST
Human NFKBIA-3340H Recombinant Human NFKBIA protein(Phe2-Leu317), His-tagged E. coli N-His
Human NFKBIA-164H Recombinant Human NFKBIA protein, His/MBP-tagged E.coli His/MBP
Human NFKBIA-28232TH Recombinant Human NFKBIA, His-tagged E.coli His
Human NFKBIA-6897H Recombinant Human Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha, His-tagged E.coli His
Human NFKBIA-437HCL Recombinant Human NFKBIA lysate HEK293 N/A
Human NFKBIA-819H Recombinant Human NFKBIA Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human NFKBIA-3784H Recombinant Human NFKBIA Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human NFKBIA-2581H Active Recombinant Human NFKBIA protein E.coli N/A
Human NFKBIA-113H Recombinant Human NFKBIA protein, GST-tagged E. coli GST
Human NFKBIA-4693H Recombinant Human NFKBIA Protein (Lys67-Glu302), N-His tagged E.coli N-His
Human CAB11587RH Rabbit Monoclonal Antibody to Human Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor, Alpha Rabbit N/A
Mouse NFKBIA-10624M Recombinant Mouse NFKBIA Protein Mammalian Cell His
Mouse Nfkbia-4398M Recombinant Mouse Nfkbia Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse NFKBIA-6039M Recombinant Mouse NFKBIA Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse NFKBIA-6039M-B Recombinant Mouse NFKBIA Protein Pre-coupled Magnetic Beads HEK293
Rat NFKBIA-3975R Recombinant Rat NFKBIA Protein Mammalian Cell His
Rat NFKBIA-3634R Recombinant Rat NFKBIA Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat NFKBIA-3634R-B Recombinant Rat NFKBIA Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque NFKBIA-3016R Recombinant Rhesus monkey NFKBIA Protein, His-tagged Mammalian Cell His
Rhesus Macaque NFKBIA-2835R-B Recombinant Rhesus Macaque NFKBIA Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque NFKBIA-2835R Recombinant Rhesus Macaque NFKBIA Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Chicken NFKBIA-1115C Recombinant Chicken NFKBIA Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • NFKBIA Related Articles
  • NFKBIA Related Research Area

NFKBIA involved in several pathways and played different roles in them. We selected most pathways NFKBIA participated on our site, such as cAMP signaling pathway, Chemokine signaling pathway, NF-kappa B signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with NFKBIA were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
cAMP signaling pathwayPPP1CC;PLCE1;PPARA;ATP1A3;GLI1;CALM2;SLC9A1;PDE4D;HTR1E
Chemokine signaling pathwayGM2506;CXCL5;CXCR3;ARRB1;SOS1;PIK3CA;PTK2;GNG13;CXCL6
NF-kappa B signaling pathwayLY96;MYD88;TRAF1;BCL2A1A;CD40;BIRC2;LTBR;BIRC3;SYK
ApoptosisBIDA;TNF;TNFRSF10C;PIK3R3A;IRF2A;CASP6L1;BIRC3;TP53;HDR
Osteoclast differentiationOSCAR;MAP2K7;NFKB2;PIK3R1;LILRB1;FCGR3A;PPP3CB;NFKBIA;FOSB
Toll-like receptor signaling pathwayIFNA7;STAT1A;TOLLIP;LBP;TLR2-1;PIK3CG;MAP2K3;CRFB2;NFKBIAA
NOD-like receptor signaling pathwayCCL2;NLRP1;NFKBIAB;MAPK14B;IL8L2;MAPK12B;TNF;BIRC3;TNFAIP3
RIG-I-like receptor signaling pathwayIFNPHI1;MAPK10;DDX3X;IFNA13;IFNA21;IFNK;MAPK8B;IFNA7;IRF7
Cytosolic DNA-sensing pathwayIFNPHI1;IL1B;POLR2F;IL18;IKBKE;Ifna15;Ifna11;ZBP1;POLR2E
T cell receptor signaling pathwayKRAS;LCP2;GRB2;GSK3B;PIK3R3;CDC42;MUC1;ICOS;AKT2
B cell receptor signaling pathwayLAT2;BANK1;RAC2;DAPP1;RAF1;MALT1;RELA;NEDD9;LILRB3
TNF signaling pathwayMMP9;MAP2K1;PIK3R2;MAP2K4;TNFRSF1A;BAG4;CASP8;AKT1;MAPK8
Neurotrophin signaling pathwaySH2B3;KIDINS220;MAPK8;PIK3CD;FOXO3;PIK3R5;SH2B1;ABL1;MAP3K5
Adipocytokine signaling pathwayMAPK10;RXRAA;TNFRSF1B;SLC2A1;MAPK8B;NFKB1;MTOR;TNF;CAMKK2
Insulin resistanceMAPK8;NFKBIAB;AKT2L;AKT2;PCK2;CREB5;SLC27A3;NR1H3;CREB3L3L
Epithelial cell signaling in Helicobacter pylori infectionMET;CXCR1;ATP6V1E1;ATP6V1D;MAPK9;MAPK10;ATP6V0C;SRC;JAM3
ShigellosisCTTN;RELA;CD44;ELMO3;NFKBIA;MAPK8;MAPK11;SRC;U2AF1
LegionellosisCLK1;MYD88;Casp3;HSPA6;HSF1;IL18;C3;EEF1A2;CXCL3
LeishmaniasisIL4;PRKCB;FCGR3B;TLR2;FCGR2A;NCF1;ITGB1;HLA-DMA;FCGR3A
Chagas disease (American trypanosomiasis)CCL2;PPP2R2A;TGFBR2;SMAD3;SMAD2;MYD88;PLCB4;GNAL;CD3E; CD3D
ToxoplasmosisMAP2K6;HSPA1B;LAMC1;LAMB3;HSPA6;NFKBIB;IL10RB;NFKB1;IRGM
Hepatitis COCLN;PPP2R2D;STAT1;PPP2R2B;CLDN9;CLDN2;MAPK8;CLDN6;CLDN14
Hepatitis BTGFBR1;IL-8;BAD;PIK3CB;IFNA6;IFNA14;TLR4;MAP2K4;TIRAP
MeaslesTLR7;Ifna11;CD209A;FAS;NFKB1;RELA;CD209C;OAS1;CCNE1
Influenza AAGFG1;TNF;TNFRSF10D;IL1B;PIK3R2;PRKCB;AKT2;MAPK3;TLR3
HTLV-I infectionPRKACA;WNT2;MAP3K3;PPP3CA;HLA-DOA;PIK3R3;ANAPC11;LTBR;RAN
Herpes simplex infectionTNFRSF1A;IL12A;TAB2;TP53;ABCB3L1;SRSF7A;BF2;MCRS1;LTA
Epstein-Barr virus infectionPOLR3E;MAP2K7;PRKACA;MAP2K4;HSPA8;POLR3B;TRAF5;SNW1;CD58
Pathways in cancerIGF1R;ITGA6;FN1;SOS1;WNT7A;VHL;PIK3CA;FGF2;F2RL3
Viral carcinogenesisHDAC8;VAC14;HIST1H2BC;HDAC1;STAT5B;HIST1H2BE;HDAC7;CDC42;HIST1H2BJ
Prostate cancerARAF;CCNE2;INS1;CREB3L2;HSP90B1;CPN1;ATF4;Ar;PIK3CG
Chronic myeloid leukemiaCBL;BAD;ARAF;MAP2K1;TGFB3;PIK3CD;SHC3;RUNX1;RAF1
Small cell lung cancerFN1;LAMC1;NFKBIA;ITGA2;NOS2;LAMB3;NFKB1;PIK3CD;PIK3CA

NFKBIA has several biochemical functions, for example, NF-kappaB binding, enzyme binding, heat shock protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by NFKBIA itself. We selected most functions NFKBIA had, and list some proteins which have the same functions with NFKBIA. You can find most of the proteins on our site.

Function Related Protein
NF-kappaB bindingSNAI1A;RNF25;HDAC1;GSK3B;EP300;FOXP3;HDAC3;BCL10;HIF1AN
enzyme bindingGSTM2;Slc4a2;CCND1;PHB;NOS1;CBX4;AKT1;UGT1A9;PARP4
heat shock protein bindingKCNJ11;HSPA1L;DNAJC7;DNAJA3A;DNAJA3;HDAC2;TOMM34;SSUH2RS1;GRXCR2
identical protein bindingSP100;DCTPP1;PAK2;ATL1;HJURP;SYT9;SDCBP;HAND1;EID3
nuclear localization sequence bindingNUP214;KPNA6;IPO5;KPNA2;IPO4;IPO13;RANBP6;KPNA3;TNPO2
protein bindingPOMP;WFS1;CHGB;FN1;PAX9;TBC1D19;NPEPL1;PCMT1;CDK5R1
protein complex bindingCEBPA;CALCA;BCAP31;SHARPIN;VHLL;CDK5RAP2;CRTAP;UQCRC2;EPCAM
transcription factor bindingSUMO1;CD34;IRF4;FHL2;YWHAZ;NAB2;HMGA1;PPARGC1A;APBB2
ubiquitin protein ligase bindingHSPA1A;TRIM28;HSPA9;BLZF1;NEDD8;WFS1;TPI1;SPG20;DIO2

NFKBIA has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NFKBIA here. Most of them are supplied by our site. Hope this information will be useful for your research of NFKBIA.

RELA

Buffington, SA; Sobotzik, JM; et al. I kappa B alpha is not required for axon initial segment assembly. MOLECULAR AND CELLULAR NEUROSCIENCE 50:1-9(2012).
Kennedy, RB; Ovsyannikova, IG; et al. Multigenic control of measles vaccine immunity mediated by polymorphisms in measles receptor, innate pathway, and cytokine genes. VACCINE 30:2159-2167(2012).
  • Q&As
  • Reviews

Q&As (6)

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What is the interaction between NFKBIA protein and NF-κB? 11/26/2022

The interaction between NFKBIA protein and NF-κB can be identified by co-immunoprecipitation, bimolecular fluorescence complementation, etc. In addition, these methods can also be used to study the interaction sites and mechanisms of action between them.

What is the effect of knocking out the NFKBIA gene on cell growth and apoptosis? 11/21/2022

Knockdown of the NFKBIA gene leads to increased cell growth arrest and apoptosis, which may be related to the aberrant activation of the NF-κB signaling pathway.

How can I study the effect of NFKBIA protein on the NF-κB signaling pathway? 04/17/2022

The effect on the expression level and activity of molecules related to the NF-κB signaling pathway can be observed by overexpressing or knocking down NFKBIA protein. In addition, studies can also be performed with specific knockout or mutant cell lines.

Is it possible to knock out NFKBIA by gene knockout? 01/08/2022

Its role in physiological and pathological processes can be studied by specifically knocking out the NFKBIA gene using specific short hairpin RNA or zinc finger nuclease technology.

How does the expression level of NFKBIA protein relate to disease? 07/07/2020

Studies have shown that the expression level of NFKBIA protein is associated with the occurrence and progression of a variety of diseases. For example, in some cancers, the expression levels of the NFKBIA protein are reduced or missing, leading to aberrant activation of the NF-κB signaling pathway.

Do NFKBIA proteins regulate the NF-κB signaling pathway? 01/21/2020

When cells are stimulated by inflammation, the NFKBIA protein is phosphorylated, causing its dissociation from NF-κB. After dissociation, NF-κB is transferred to the nucleus and activates the transcription of specific genes. In addition, NFKBIA protein can further regulate the activity of NF-κB by interacting with other proteins.

Customer Reviews (3)

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Reviews
07/04/2022

    I am very happy with the results, they performed very well in my experiments.

    05/26/2022

      It has high sensitivity and strong specificity.

      04/07/2022

        It is easy to use and requires little preparation before the experiment.

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