NFKBIA
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Official Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cell extracts inhibitor, alpha
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Overview
IκBα (Inhibitor of Nuclear Factor (NF)-κB α isoform) is a member of the IκB protein family with a MW of 40 kDa. IκBα is ubiquitously expressed among mammals. Located at IκBα's N-terminus is a signal response domain which contains serine residues that can be phosphorylated, while the C-terminus contains a PEST domain, a common feature among proteins with high turnover rates. As with all members of the IκB family, IκBα possesses ankyrin repeats approximately 33 amino acids in length, which mediate binding to the Rel homology region of NF-κB. The interaction of IκBα with NF-κB masks the nuclear localization sequence of NF-κB, preventing NF-κB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF-κB through the degradation of IκBα. These stimuli include the proinflammatory cytokines TNF-α and IL-1β, chemokines, PMA, growth factors, LPS, UV irradiation, and viral infection, as well as various chemical and physical stresses. The series of events leading to this liberation is well-defined. In response to stimulus, Ser32/36 of IκBα are phosphorylated, which provides a signal for IκBα E3 ligase, a protein complex composed of SKP-1, Cul-1, Roc1, and Fbw1. IκBα E3 ligase polyubiquitinates IκBα at lysine residues 21 and 22, and the polyubiquitinated IκBα is then targeted to the 26S proteasome for degradation. Liberated NF-κB is transported across the nuclear membrane, where it activates transcription by binding to the consensus sequence GGRNNYYCC, which is found in the promoter regions of a large number of genes including IL-6, VEGF, VCAM-1, ICAM-1, HIV long terminal repeat, and many others. The initial event which targets IκBα for degradation is phosphorylation of Ser32/36. This phosphorylation is catalyzed by a protein complex known as IKK (IκB kinase). IKK contains two kinase subunits designated IKKα (MW=85 kDa) and IKKβ (MW=87 kDa), and scaffold protein designated IKKγ/NEMO (MW=48 kDa). The kinetic properties of IKK are regulated by complex formation as well as by phosphorylation events catalyzed by upstream kinases including members of the MAPK cascade, the SAPK/JNK cascade and NIK. Through its regulation of NF-κB, IκBα controls immune and inflammatory responses, cell division, and apoptosis. Numerous disease states including arthritis, asthma, and inflammatory bowel disease are associated with loss of IκBα regulation. The importance of regulation of IκBα in cancer is underscored by the observation that multiple myelomas often possess polymorphisms at IκBα regulatory sites, and certain symptoms can be ameliorated using the proteasome inhibitor PS-341 which favors the sequestration of NF-κB in the cytoplasm. -
Synonyms
IKBA; MAD-3; NFKBI; I-kappa-B-alpha; IkappaBalpha; NF-kappa-B inhibitor alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells;
- Recombinant Proteins
- Cell & Tissue Lysates
- Antibody
- Protein Pre-coupled Magnetic Beads
- Chicken
- Human
- Mouse
- Rat
- Rhesus Macaque
- E. coli
- E.coli
- HEK293
- HEK293T
- Mammalian Cell
- Rabbit
- GST
- His
- His (Fc)
- Avi
- His|MBP
- Myc
- DDK
- Myc|DDK
- N/A
- N
- Involved Pathway
- Protein Function
- Interacting Protein
- NFKBIA Related Articles
- NFKBIA Related Research Area
NFKBIA involved in several pathways and played different roles in them. We selected most pathways NFKBIA participated on our site, such as cAMP signaling pathway, Chemokine signaling pathway, NF-kappa B signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with NFKBIA were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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cAMP signaling pathway | ATP1A1;MLLT4;PDE4C;AKT1;GPR81;LOC100328615;FXYD1;ATP1A3;EP300 |
Chemokine signaling pathway | ARRB2;CXCR5;NRAS;ARRB1;NFKB1;GNG4;GRK7;PIK3R1;CCR3 |
NF-kappa B signaling pathway | ZAP70;PIDD1;TRAF1;PLCG1;TRAF6;PLCG2;MAP3K7;CFLAR;TNFSF14 |
Apoptosis | BAXA;PRKAR2AB;CASP8;Fasl;DIABLOB;PANK1A;PIK3R2;CTNNB2;ENDOG |
Osteoclast differentiation | IFNGR2;LILRB3;LCK;TNFRSF11B;TGFB2;PIK3CD;FHL2;TNFSF11;cgr2b |
Toll-like receptor signaling pathway | IRAK4;MAP3K7;CCL4;IL6;IFNA4;TLR4BB;PIK3R3;RAC1;IFNA3 |
NOD-like receptor signaling pathway | CCL5;NLRP3;CASP8;HSP90AA1.1;MAPK12;CXCL8B.3;MAPK14A;CASP1;NFKBIA |
RIG-I-like receptor signaling pathway | IFNA12;IFNB1;TRADD;TBK1;ISG15;IFNA6;RELA;ATG5;RIPK1 |
Cytosolic DNA-sensing pathway | IFNA17;Ifna15;POLR3H;IFN-a;CCL5;NFKBIAB;IFNB1;IKBKG;NFKBIAA |
T cell receptor signaling pathway | PPP3R1;IKBKB;CD28;FYN;PAK7;VAV2;PIK3R5;PRKCQ;PIK3CD |
B cell receptor signaling pathway | LCP2A;SYK;Cd79a, Cd79b;PPP3R1;VAV1;AKT1;LYN;PIK3R1;BLNK |
TNF signaling pathway | TAB2;RIPK1;CXCL2;CFLAR;AKT2;NFKBIA;NFKB1;PIK3R3;RPS6KA5 |
Neurotrophin signaling pathway | NRAS;GRB2;PIK3R1;MAPK8;SHC3;MAP2K1;RHOA;PIK3R5;KRAS |
Adipocytokine signaling pathway | SLC2A1;ACSL4;SLC2A4;PRKAG3B;IKBKG;RXRA;NFKB1;RXRBA;IRS2 |
Insulin resistance | PPP1CB;AKT2L;SOCS3B;PTENA;PRKAA2;MTOR;PPP1CA;PPARAB;PTPN1 |
Epithelial cell signaling in Helicobacter pylori infection | PAK1;ATP6V1G3;ATP6V1D;CXCR1;CXCL1;MAPK11;MAP3K14;MAPK12;ATP6V0A4 |
Shigellosis | ELMO2;MAPK13;ELMO3;PFN2;IKBKG;U2AF1;PFN3;MAPK9;NFKBIB |
Legionellosis | HSPA2;IL18;BNIP3;EEF1G;HSPD1;HSPA1B;CR1;SAR1B;Casp3 |
Leishmaniasis | HLA-DQA1;HLA-DQB1;HLA-DMB;MAPK11;FCGR3A;C3;FOS;Itgam&Itgb2;NFKBIB |
Chagas disease (American trypanosomiasis) | C1QA;PIK3R5;TGFBR2;IFNGR2;PIK3CD;GNAQ;IFNB1;RELA;FOS |
Toxoplasmosis | CYCS;TGFB1;PIK3R1;LDLR;Toxoplasma Gondii Major Surface Antigen P30;MYD88;LAMB4;LAMB1;LAMA4 |
Hepatitis C | IFNA5;TBK1;IFNA8;TRP53;AKT1;CLDN2;EIF2AK1;PIK3CB;TNFRSF1A |
Hepatitis B | IL-8;MYD88;CCNA1;IFNA12;NFATC4;AKT2;YWHAZ;EGR3;BCL2 |
Measles | TNFRSF10C;CLEC4M;IFNA2;HSPA1B;TNFRSF10D;NFKB1;TLR7;CD209C;HSPA2 |
Influenza A | HLA-DRB3;BLA;RNASEL;CCL5;PABPN1L;HLA-DRB5;NLRX1;IFNA12;PIK3R3 |
HTLV-I infection | VDAC3;WNT2B;PIK3R5;IL1R2;NFATC1;PIK3CG;XBP1;FZD6;RELB |
Herpes simplex infection | SRSF7;STAT1A;SP100;HLA-C;PER1;IL1B;IFNA8;CUL1;MED8 |
Epstein-Barr virus infection | IL10RB;EP300;RAN;LYN;POLR2L;HLA-F;PSMD2;HLA-A;MAP2K6 |
Pathways in cancer | IL-8;DAPK3;AGTR1B;FZD3;FAS;KITLG;PIK3CB;CBL;MSH6 |
Viral carcinogenesis | PXN;CREB3L2;PIK3R3;VAC14;TBPL2;HDAC11;YWHAG;HDAC8;SNW1 |
Prostate cancer | PDGFRB;SOS1;Ar;CASP9;GRB2;MTOR;PDGFA;EP300;SMARCC2 |
Chronic myeloid leukemia | HDAC2;NRAS;MAP2K1;PIK3CD;RUNX1;CBLC;AKT1;PIK3CB;IKBKB |
Small cell lung cancer | BCL2;LAMB4;NFKB1;PIK3CB;TRP53;ITGA2;FHIT;NOS2;TRAF5 |
NFKBIA has several biochemical functions, for example, NF-kappaB binding, enzyme binding, heat shock protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by NFKBIA itself. We selected most functions NFKBIA had, and list some proteins which have the same functions with NFKBIA. You can find most of the proteins on our site.
Function | Related Protein |
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NF-kappaB binding | NFKBIA;BCL10;HSPA6;ANKRD42;SNAI1A;HDAC1;TAF4B;CDKN2A;BRMS1 |
enzyme binding | CASP2;Ar;RAD9;C6orf211;Slc4a2;TSPYL1;MST1R;NKX2-1;CDC20 |
heat shock protein binding | DLST;BCOR;LIMK1;PACRG;FKBP4;HSPA1A;HSPA9;DNAJC7;DNAJA4 |
identical protein binding | ZNF3;MCM6;AGER;MPST;HAND2;RGMB;RIPK3;KCTD17;TRAF2 |
nuclear localization sequence binding | IPO5;KPNB1;TNPO2;TNPO1;KPNA3;IPO13;KPNA5;KPNB3;TNPO3 |
protein binding | RPL22;FAM167A;MED28;XAGE2;DNA2;LMAN1;CLDN16;CXorf48;CCNB2 |
protein complex binding | ANKRD54;KRAS;STRN4;NCKAP1;HSPD1;SH2B3;GNB2;PDX1;TRADD |
transcription factor binding | LMO4;SMARCA4;NIF3L1;PELP1;TFDP1B;H2-AB1;NKX2-5;JUNBA;HIF1A |
ubiquitin protein ligase binding | MAGEC2;RBX1;CDKN1A;C10orf46;SMAD3;NEDD8;VCL;HSPD1;STX8 |
NFKBIA has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NFKBIA here. Most of them are supplied by our site. Hope this information will be useful for your research of NFKBIA.
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Q&As (6)
Ask a questionThe interaction between NFKBIA protein and NF-κB can be identified by co-immunoprecipitation, bimolecular fluorescence complementation, etc. In addition, these methods can also be used to study the interaction sites and mechanisms of action between them.
Knockdown of the NFKBIA gene leads to increased cell growth arrest and apoptosis, which may be related to the aberrant activation of the NF-κB signaling pathway.
The effect on the expression level and activity of molecules related to the NF-κB signaling pathway can be observed by overexpressing or knocking down NFKBIA protein. In addition, studies can also be performed with specific knockout or mutant cell lines.
Its role in physiological and pathological processes can be studied by specifically knocking out the NFKBIA gene using specific short hairpin RNA or zinc finger nuclease technology.
Studies have shown that the expression level of NFKBIA protein is associated with the occurrence and progression of a variety of diseases. For example, in some cancers, the expression levels of the NFKBIA protein are reduced or missing, leading to aberrant activation of the NF-κB signaling pathway.
When cells are stimulated by inflammation, the NFKBIA protein is phosphorylated, causing its dissociation from NF-κB. After dissociation, NF-κB is transferred to the nucleus and activates the transcription of specific genes. In addition, NFKBIA protein can further regulate the activity of NF-κB by interacting with other proteins.
Customer Reviews (3)
Write a reviewI am very happy with the results, they performed very well in my experiments.
It has high sensitivity and strong specificity.
It is easy to use and requires little preparation before the experiment.
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