PHF8
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Official Full Name
PHD finger protein 8 -
Overview
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. -
Synonyms
PHF8;PHD finger protein 8;histone lysine demethylase PHF8;JHDM1F;jumonji C domain containing histone demethylase 1F;KIAA1111;ZNF422;jumonji C domain-containing histone demethylase 1F;MRXSSD;DKFZp686E0868
Recombinant Proteins
- Mouse
- Human
- Zebrafish
- E.coli
- Sf9 Cells
- Mammalian Cells
- HEK293
- His
- Flag
- Non
- Avi
- Fc
| Cat.# | Product name | Source (Host) | Species | Tag | Protein Length | Price |
|---|---|---|---|---|---|---|
| Phf8-751M | Recombinant Mouse Phf8 Protein, His-tagged | E.coli | Mouse | His | Phe195~Lys351 | |
| PHF8-61H |
Active Recombinant Human PHF8, FLAG-tagged
|
Sf9 Cells | Human | Flag |
|
|
| PHF8-12733M | Recombinant Mouse PHF8 Protein | Mammalian Cells | Mouse | His |
|
|
| PHF8-62H | Recombinant Human PHF8, GST-tagged | E.coli | Human | Non | 1-447 a.a. |
|
| PHF8-63H | Recombinant Human PHF8, GST-tagged | E.coli | Human | Non | 80-447 a.a. |
|
| PHF8-7972Z | Recombinant Zebrafish PHF8 | Mammalian Cells | Zebrafish | His |
|
|
| PHF8-0537H | Recombinant Human PHF8 Protein (A38-N483), Tag Free | E.coli | Human | Non | A38-N483 |
|
| PHF8-0538H | Recombinant Human PHF8 Protein (A38-N483), His tagged | E.coli | Human | His | A38-N483 |
|
| PHF8-6695M | Recombinant Mouse PHF8 Protein, His (Fc)-Avi-tagged | HEK293 | Mouse | Avi&Fc&His |
|
|
| PHF8-6695M-B | Recombinant Mouse PHF8 Protein Pre-coupled Magnetic Beads | HEK293 | Mouse |
|
Involved Pathway
PHF8 involved in several pathways and played different roles in them. We selected most pathways PHF8 participated on our site, such as Cell Cycle,Cell Cycle, Mitotic,Chromatin modifying enzymes, which may be useful for your reference. Also, other proteins which involved in the same pathway with PHF8 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Condensation of Prophase Chromosomes | NCAPD3,SMC2,SET,SETB,H3F3D,NCAPH2,MCPH1,SMC4,NCAPG2,H3F3B.1 |
| Chromatin modifying enzymes | PRMT7,ELP4,KDM1B,SMARCB1A,MSL2,KDM2AA,CHD4A,KAT5,PHF15,AEBP2 |
| Chromatin organization | KDM4C,PBRM1,JHDM1D,KDM1B,PHF15,BRPF1,BRD1,ARID1A,PRDM16,ENY2 |
| Cell cycle | YWHABB,CCNA2,BRE,LIN52,GMNN,SMC1AL,ORC4L,GADD45AA,CDC20,DSN1 |
| M Phase | CENPO,VRK2,CDCA5,NIPBLA,RCC2,DSN1,NDEL1A,NCAPH,NCAPG2,SET |
| HDMs demethylate histones | KDM4A,KDM2AA,KDM4B,KDM2A,KDM1A,KDM5BA,KDM4D,KDM5D,KDM4C,PHF2 |
| Cell Cycle, Mitotic | DYNC1H1,CNEP1R1,CDKN2D,E2F3,NUP35,CENPK,KIF2B,USO1,HIST2H3C,LIN9 |
| Mitotic Prophase | SET,VRK2,DULLARD,MASTL,SMC4,NEK9,ARPP19A,CTDNEP1B,SETB,TMEM188 |
Protein Function
PHF8 has several biochemical functions, for example, chromatin binding,histone demethylase activity,histone demethylase activity (H3-K27 specific). Some of the functions are cooperated with other proteins, some of the functions could acted by PHF8 itself. We selected most functions PHF8 had, and list some proteins which have the same functions with PHF8. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| chromatin binding | ASH1L,SVEP1,SOD3,ATOH1A,MEF2C,CREBBP,NFKB1,EZH1,KLF6A,NKX2 |
| histone demethylase activity (H3-K27 specific) | KDM6B,KDM6A,JHDM1D,KDM7AB,KDM6BB |
| histone demethylase activity (H4-K20 specific) | HIST1H4H,HIST1H4K,HIST1H4E,HIST1H4F,HIST2H4A,HIST1H4B,HIST1H4L,HIST1H4I,HIST1H4A,HIST1H4J |
| iron ion binding | CYP2P6,P3H2,KDM3A,HBG2,CYP2AD3,CYP2K17,Agmo,EGLN3,CYP2B6,CYP2AA7 |
| methylated histone binding | JHDM1D,PHF1,RAG2,L3MBTL2,ING2,FAM156A,ZMYND11,PHF13,MTF2,WDR92 |
| histone demethylase activity (H3-K9 specific) | PHF2,KDM4D,KDM4C,JHDM1D,KDM1A,KDM7AB |
| histone demethylase activity (H3-K36 specific) | KDM2A,JHDM1D,KDM2B,C14orf169,KDM8,KDM4A,JMJD5 |
| zinc ion binding | TRAF2,ADAMTS20,MMP23BB,ADH8B,BRPF1,TRIM15,S100A7,RBBP6,PHF13,OAS1 |
| protein binding | PPP2R5B,SOST,GLB1,KCTD15,FBXO25,CD28,KIF20A,PSMB3,PPHLN1,NT5C2 |
Interacting Protein
PHF8 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PHF8 here. Most of them are supplied by our site. Hope this information will be useful for your research of PHF8.
Dlg4;PML-RAR;h3_peptide_2-18;rarb_human_gene;H2AFY;CENPB;EIF6;HIST1H3D;MRPS35;MRPS15;XPC;TRIM26;TRIP12;PLEC;HIST2H2BE;DNTTIP2;RIF1;BRAT1;NOL8;MB21D1
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References
- Shi, G; Wu, M; et al. PHD Finger Protein 2 (PHF2) Represses Ribosomal RNA Gene Transcription by Antagonizing PHF Finger Protein 8 (PHF8) and Recruiting Methyltransferase SUV39H1. JOURNAL OF BIOLOGICAL CHEMISTRY 289:29691-29700(2014).
- Lubula, MY; Poplawaski, A; et al. Crystallization and preliminary X-ray diffraction analysis of the BRPF1 bromodomain in complex with its H2AK5ac and H4K12ac histone-peptide ligands. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70:1389-1393(2014).
