Recombinant Human Pim-1 Oncogene, GST-tagged, Active

• Cat.No.: PIM1-392H
• Product Overview: Recombinant human full-length PIM1 was expressed by baculovirus inSf9 cells usingan N-terminal GST tag. MW=62 kDa.

Specification

• Cat. No.: PIM1-392H
• Description: PIM1 is a proto-oncogene that belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, PIM1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of PIM1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Expression of PIM1 is mediated through activation of the JAK/STAT pathway.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PIM1 pim-1 oncogene [ Homo sapiens ]
• Synonyms: PIM1; pim-1 oncogene; PIM; Proto-oncogene serine/threonine-protein kinase Pim-1; EC 2.7.11.1
• Gene ID: 5292
• mRNA Refseq: NM_002648
• Protein Refseq: NP_002639
• UniProt ID: P11309
• Chromosome Location: 6p21
• MIM: 164960
• Pathway: Acute myeloid leukemia; Jak-STAT signaling pathway
• Function: ATP binding; nucleotide binding; transferase activity; manganese ion binding; protein serine/threonine kinase activity; metal ion binding; transcription factor binding

Reviews

01/30/2022

With the combination of this protein reagent's instructions, I can easily accomplish experimental design and operation.

04/29/2020

With consistent and reliable results, this protein reagent is ideal for research projects that require replicability.

01/02/2020

Following the use of this protein reagent, the reproducibility of my experiments has significantly improved, and I am highly satisfied.

Q&As

What are the main substrates of PIM1 and how does their interaction affect cellular processes?

PIM1 phosphorylates a variety of substrates, including BAD, c-Myc, and eIF4B. Phosphorylation of BAD inhibits its pro-apoptotic function, while phosphorylation of c-Myc and eIF4B enhances their transcriptional and translational activities, promoting cell survival and protein synthesis, respectively.

How is PIM1 activity regulated by signaling pathways?

PIM1 activity is regulated by several signaling pathways, including the PI3K/AKT and JAK/STAT pathways. Activation of these pathways leads to the phosphorylation and activation of PIM1, while inhibition of these pathways can result in reduced PIM1 activity.

What is the potential of PIM1 as a prognostic marker or therapeutic target in different cancer types?

Further research is needed to assess the potential of PIM1 as a prognostic marker and therapeutic target in different cancer types. Understanding the specific roles of PIM1 in various cancers and its interactions with other signaling pathways will help determine its clinical relevance and potential utility in personalized cancer treatment strategies.

What role does PIM1 play in cancer development?

PIM1 has been implicated in promoting cell proliferation, survival, and resistance to apoptosis, contributing to cancer development. Its overexpression has been observed in various cancer types and is associated with poor prognosis. PIM1 also interacts with oncogenic signaling pathways, further promoting tumor growth and progression.

Are there any potential therapeutic strategies targeting PIM1?

Several small molecule inhibitors targeting PIM1 kinase activity have been developed and show promise as potential therapeutics. These inhibitors can selectively inhibit PIM1 activity and have demonstrated anti-tumor effects in preclinical studies. Combination therapy with PIM1 inhibitors and other targeted drugs may also be a viable approach for treating PIM1-driven cancers.

How does PIM1 regulate cell cycle progression and proliferation?

PIM1 regulates cell cycle progression and proliferation by phosphorylating key substrates, such as p27 and CDC25A, which control the activity of cyclin-dependent kinases (CDKs). This phosphorylation promotes the degradation of p27 and stabilizes CDC25A, leading to enhanced CDK activity and cell cycle progression.

How is PIM1 expression regulated at the transcriptional level?

PIM1 expression can be regulated by various factors, including cytokines, growth factors, and oncogenic signals. These factors can activate specific transcription factors, such as STAT3 and NF-κB, which bind to the PIM1 promoter and enhance its transcriptional activity, resulting in increased PIM1 expression.