Recombinant Human PIM1, His-tagged
Cat.No. : | PIM1-7553H |
Product Overview : | Recombinant human Pim1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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Antigen Description : | PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
Molecular Mass : | 31.4 kDa (274aa) confirmed by MALDI-TOF |
Protein length : | 38-290aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MYQVGPLLGS GGFGSVYSGI RVSDNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSGFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEELARS FFWQVLEAVR HCHNCGVLHR DIKDENILID LNRGELKLID FGSGALLKDT VYTDFDGTRV YSPPEWIRYH RYHGRSAAVW SLGILLYDMV CGDIPFEHDE EIIRGQVFFR QRVSSECQHL IRWCLALRPS DRPTFEEIQN HPWM |
Purity : | >90% as determined by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | PIM1 pim-1 oncogene [ Homo sapiens ] |
Official Symbol : | PIM1 |
Synonyms : | PIM1; pim-1 oncogene; PIM; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; |
Gene ID : | 5292 |
mRNA Refseq : | NM_001243186 |
Protein Refseq : | NP_001230115 |
MIM : | 164960 |
UniProt ID : | P11309 |
Chromosome Location : | 6p21 |
Pathway : | Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Role of Calcineurin-dependent NFAT signaling in lymphocytes, organism-specific biosystem; |
Function : | ATP binding; manganese ion binding; metal ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transcription factor binding; |
Products Types
◆ Recombinant Protein | ||
PIM1-1680H | Recombinant Human PIM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PIM1-4125R | Recombinant Rat PIM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Pim1-4868M | Recombinant Mouse Pim1 Protein, Myc/DDK-tagged | +Inquiry |
PIM1-0920H | Recombinant Human PIM1 Protein (A14-K313), His tagged | +Inquiry |
PIM1-0919H | Recombinant Human PIM1 Protein (A14-K313), Tag Free | +Inquiry |
◆ Lysates | ||
PIM1-3181HCL | Recombinant Human PIM1 293 Cell Lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewWith the combination of this protein reagent's instructions, I can easily accomplish experimental design and operation.
With consistent and reliable results, this protein reagent is ideal for research projects that require replicability.
Following the use of this protein reagent, the reproducibility of my experiments has significantly improved, and I am highly satisfied.
Q&As (7)
Ask a questionPIM1 phosphorylates a variety of substrates, including BAD, c-Myc, and eIF4B. Phosphorylation of BAD inhibits its pro-apoptotic function, while phosphorylation of c-Myc and eIF4B enhances their transcriptional and translational activities, promoting cell survival and protein synthesis, respectively.
PIM1 activity is regulated by several signaling pathways, including the PI3K/AKT and JAK/STAT pathways. Activation of these pathways leads to the phosphorylation and activation of PIM1, while inhibition of these pathways can result in reduced PIM1 activity.
Further research is needed to assess the potential of PIM1 as a prognostic marker and therapeutic target in different cancer types. Understanding the specific roles of PIM1 in various cancers and its interactions with other signaling pathways will help determine its clinical relevance and potential utility in personalized cancer treatment strategies.
PIM1 has been implicated in promoting cell proliferation, survival, and resistance to apoptosis, contributing to cancer development. Its overexpression has been observed in various cancer types and is associated with poor prognosis. PIM1 also interacts with oncogenic signaling pathways, further promoting tumor growth and progression.
Several small molecule inhibitors targeting PIM1 kinase activity have been developed and show promise as potential therapeutics. These inhibitors can selectively inhibit PIM1 activity and have demonstrated anti-tumor effects in preclinical studies. Combination therapy with PIM1 inhibitors and other targeted drugs may also be a viable approach for treating PIM1-driven cancers.
PIM1 regulates cell cycle progression and proliferation by phosphorylating key substrates, such as p27 and CDC25A, which control the activity of cyclin-dependent kinases (CDKs). This phosphorylation promotes the degradation of p27 and stabilizes CDC25A, leading to enhanced CDK activity and cell cycle progression.
PIM1 expression can be regulated by various factors, including cytokines, growth factors, and oncogenic signals. These factors can activate specific transcription factors, such as STAT3 and NF-κB, which bind to the PIM1 promoter and enhance its transcriptional activity, resulting in increased PIM1 expression.
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