Recombinant Human PIM1 protein, His-tagged
Cat.No. : | PIM1-2714H |
Product Overview : | Recombinant Human PIM1 protein(244-313 aa), fused to His tag, was expressed in E. coli. |
Availability | February 17, 2025 |
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Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole. |
Protein length : | 244-313 aa |
AA Sequence : | HDEEIIRGQVFFRQRVSSECQHLIR WCLALRPSDRPTFEEIQNHPWMQDV LLPQETAEIHLHSLSPGPSK |
Purity : | 95%, by SDS-PAGE with Coomassie Brilliant Blue staining. |
Storage : | Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles. |
Reconstitution : | Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage. Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage. |
Gene Name : | PIM1 pim-1 oncogene [ Homo sapiens ] |
Official Symbol : | PIM1 |
Synonyms : | PIM1; pim-1 oncogene; PIM; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; |
Gene ID : | 5292 |
mRNA Refseq : | NM_001243186 |
Protein Refseq : | NP_001230115 |
MIM : | 164960 |
UniProt ID : | P11309 |
Products Types
◆ Recombinant Protein | ||
Pim1-4868M | Recombinant Mouse Pim1 Protein, Myc/DDK-tagged | +Inquiry |
PIM1-1680H | Recombinant Human PIM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PIM1-4125R | Recombinant Rat PIM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PIM1-0919H | Recombinant Human PIM1 Protein (A14-K313), Tag Free | +Inquiry |
PIM1-0920H | Recombinant Human PIM1 Protein (A14-K313), His tagged | +Inquiry |
◆ Lysates | ||
PIM1-3181HCL | Recombinant Human PIM1 293 Cell Lysate | +Inquiry |
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Customer Reviews (3)
Write a reviewWith the combination of this protein reagent's instructions, I can easily accomplish experimental design and operation.
With consistent and reliable results, this protein reagent is ideal for research projects that require replicability.
Following the use of this protein reagent, the reproducibility of my experiments has significantly improved, and I am highly satisfied.
Q&As (7)
Ask a questionPIM1 phosphorylates a variety of substrates, including BAD, c-Myc, and eIF4B. Phosphorylation of BAD inhibits its pro-apoptotic function, while phosphorylation of c-Myc and eIF4B enhances their transcriptional and translational activities, promoting cell survival and protein synthesis, respectively.
PIM1 activity is regulated by several signaling pathways, including the PI3K/AKT and JAK/STAT pathways. Activation of these pathways leads to the phosphorylation and activation of PIM1, while inhibition of these pathways can result in reduced PIM1 activity.
Further research is needed to assess the potential of PIM1 as a prognostic marker and therapeutic target in different cancer types. Understanding the specific roles of PIM1 in various cancers and its interactions with other signaling pathways will help determine its clinical relevance and potential utility in personalized cancer treatment strategies.
PIM1 has been implicated in promoting cell proliferation, survival, and resistance to apoptosis, contributing to cancer development. Its overexpression has been observed in various cancer types and is associated with poor prognosis. PIM1 also interacts with oncogenic signaling pathways, further promoting tumor growth and progression.
Several small molecule inhibitors targeting PIM1 kinase activity have been developed and show promise as potential therapeutics. These inhibitors can selectively inhibit PIM1 activity and have demonstrated anti-tumor effects in preclinical studies. Combination therapy with PIM1 inhibitors and other targeted drugs may also be a viable approach for treating PIM1-driven cancers.
PIM1 regulates cell cycle progression and proliferation by phosphorylating key substrates, such as p27 and CDC25A, which control the activity of cyclin-dependent kinases (CDKs). This phosphorylation promotes the degradation of p27 and stabilizes CDC25A, leading to enhanced CDK activity and cell cycle progression.
PIM1 expression can be regulated by various factors, including cytokines, growth factors, and oncogenic signals. These factors can activate specific transcription factors, such as STAT3 and NF-κB, which bind to the PIM1 promoter and enhance its transcriptional activity, resulting in increased PIM1 expression.
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