Recombinant Human PIM1 protein, GST-tagged

Cat.No. : PIM1-301405H
Product Overview : Recombinant Human PIM1 (244-313 aa) protein, fused to GST tag, was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
  • Download
Source : E. coli
Species : Human
Tag : GST
Form : The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization.
Protein length : His244-Lys313
AA Sequence : HDEEIIRGQVFFRQRVSSECQHLIR WCLALRPSDRPTFEEIQNHPWMQDV LLPQETAEIHLHSLSPGPSK
Purity : 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Stability : Store for up to 12 months at -20°C to -80°C as lyophilized powder.
Storage : Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Gene Name : PIM1 pim-1 oncogene [ Homo sapiens ]
Official Symbol : PIM1
Synonyms : PIM1; pim-1 oncogene; PIM; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1;
Gene ID : 5292
mRNA Refseq : NM_001243186
Protein Refseq : NP_001230115
MIM : 164960
UniProt ID : P11309

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
What are the main substrates of PIM1 and how does their interaction affect cellular processes? 01/28/2022

PIM1 phosphorylates a variety of substrates, including BAD, c-Myc, and eIF4B. Phosphorylation of BAD inhibits its pro-apoptotic function, while phosphorylation of c-Myc and eIF4B enhances their transcriptional and translational activities, promoting cell survival and protein synthesis, respectively.

How is PIM1 activity regulated by signaling pathways? 05/01/2021

PIM1 activity is regulated by several signaling pathways, including the PI3K/AKT and JAK/STAT pathways. Activation of these pathways leads to the phosphorylation and activation of PIM1, while inhibition of these pathways can result in reduced PIM1 activity.

What is the potential of PIM1 as a prognostic marker or therapeutic target in different cancer types? 11/24/2020

Further research is needed to assess the potential of PIM1 as a prognostic marker and therapeutic target in different cancer types. Understanding the specific roles of PIM1 in various cancers and its interactions with other signaling pathways will help determine its clinical relevance and potential utility in personalized cancer treatment strategies.

What role does PIM1 play in cancer development? 02/13/2020

PIM1 has been implicated in promoting cell proliferation, survival, and resistance to apoptosis, contributing to cancer development. Its overexpression has been observed in various cancer types and is associated with poor prognosis. PIM1 also interacts with oncogenic signaling pathways, further promoting tumor growth and progression.

Are there any potential therapeutic strategies targeting PIM1? 01/23/2018

Several small molecule inhibitors targeting PIM1 kinase activity have been developed and show promise as potential therapeutics. These inhibitors can selectively inhibit PIM1 activity and have demonstrated anti-tumor effects in preclinical studies. Combination therapy with PIM1 inhibitors and other targeted drugs may also be a viable approach for treating PIM1-driven cancers.

How does PIM1 regulate cell cycle progression and proliferation? 03/17/2017

PIM1 regulates cell cycle progression and proliferation by phosphorylating key substrates, such as p27 and CDC25A, which control the activity of cyclin-dependent kinases (CDKs). This phosphorylation promotes the degradation of p27 and stabilizes CDC25A, leading to enhanced CDK activity and cell cycle progression.

How is PIM1 expression regulated at the transcriptional level? 02/16/2016

PIM1 expression can be regulated by various factors, including cytokines, growth factors, and oncogenic signals. These factors can activate specific transcription factors, such as STAT3 and NF-κB, which bind to the PIM1 promoter and enhance its transcriptional activity, resulting in increased PIM1 expression.

Customer Reviews (3)

Write a review
Reviews
01/30/2022

    With the combination of this protein reagent's instructions, I can easily accomplish experimental design and operation.

    04/29/2020

      With consistent and reliable results, this protein reagent is ideal for research projects that require replicability.

      01/02/2020

        Following the use of this protein reagent, the reproducibility of my experiments has significantly improved, and I am highly satisfied.

        Ask a Question for All PIM1 Products

        Required fields are marked with *

        My Review for All PIM1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends