Recombinant Full Length Human PIM1 Protein, C-Flag-tagged

• Cat.No.: PIM1-959HFL
• Product Overview: Recombinant Full Length Human PIM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.5 kDa
• AA Sequence: MLLSKINSLAHLRAAPCNDLHATKL APGKEKEPLESQYQVGPLLGSGGFG SVYSGIRVSDNLPVAIKHVE KDRISDWGELPNGTRVPMEVVLLKK VSSGFSGVIRLLDWFERPDSFVLIL ERPEPVQDLFDFITERGALQ EELARSFFWQVLEAVRHCHNCGVLH RDIKDENILIDLNRGELKLIDFGSG ALLKDTVYTDFDGTRVYSPP EWIRYHRYHGRSAAVWSLGILLYDM VCGDIPFEHDEEIIRGQVFFRQRVS SECQHLIRWCLALRPSDRPT
  FEEIQNHPWMQDVLLPQETAEIHLH SLSPGPSKTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Jak-STAT signaling pathway

Gene Information

• Gene Name: PIM1 Pim-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: PIM1
• Synonyms: PIM
• Gene ID: 5292
• mRNA Refseq: NM_002648.4
• Protein Refseq: NP_002639.1
• MIM: 164960
• UniProt ID: P11309