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Recombinant Human Pim-1 Oncogene, His-tagged

Cat.No. : PIM1-1288H
Product Overview : Recombinant humanfull length PIM1, His-tagged. The proto-oncogene PIM1 is upregulated inprostate cancer. No special measures were taken to activate this kinase.
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Cat. No. : PIM1-1288H
Description : PIM1 is aproto-oncogene which encodes for the serine/threonine kinase of the samename. The PIM1 oncogene was first described in relation to murine T-celllymphomas, as it was the locus most frequently activated by the Moloney murineleukemia virus. Subsequently, the oncogene has been implicated in multiplehuman cancers, including prostate cancer, acute myeloid leukemia and otherhematopoietic malignancies.
Source : Insect cells
Species : Human
Form : Liquid in 50 mMTris, pH 7.5 + 500 mM NaCl + 0.5 mM EDTA + 0.05% Triton X-100 + 2 mM DTT +50% glycerol.
Concentration : 0.18 mg/mL totalprotein as measured using the Bradford protein assay with BSA as a standard.
Molecular Weight : 40.7 kDa
Purity : 90% as determined bya Coomassie blue-stained SDS-PAGE gel
Mass Spectrometry : The protein wasdigested with trypsin followed by LC-MS/MS. The resulting MS/MS data verifiedPIM1 identity from 8 peptides that covered 13% of the amino acid sequence ofthe recombinant protein.
Assay Conditions : The enzyme waspre-diluted in enzyme dilution buffer and assayed in 50 mM Tris (pH 7.5), 10mM MgCl2, 2 mM DTT, 0.025% Triton® X-100, 200 µM ATP, 100 µM PIM1 peptidesubstrate and trace [32P]-γ-ATP for 10 minutes at 30°C.
Specific Activity : 8680 nmole ofphosphate transferred to PIM1 peptide substrate (AKKRRLSA) per minute per mgof total protein at 30°C. Activity determined at a final proteinconcentration of 0.083 µg/mL.
Dilution Buffer : 20 mM Tris (pH 7.5),0.05% Triton® X-100, 0.1 mg/mL BSA, 0.5 mM Na3VO4, 2 mM DTT, 10% Glycerol.
Storage Buffer : 50 mM Tris (pH 7.5),500 mM NaCl, 0.5 mM EDTA, 0.05% Triton® X-100, 2 mM DTT, 50% Glycerol.
Storage : Store at -80°C. Atfirst use, aliquot and store at -80°C to avoid multiple freeze-thaws. Ifproperly stored at -80°C, this product is stable for 6 months from date ofpurchase. This protein comes out of solution at low salt concentrations.Avoid repeated freeze-thaw cycles.
OfficialSymbol : PIM1
Gene Name : PIM1 pim-1 oncogene [ Homo sapiens ]
Synonyms : PIM1; pim-1oncogene; PIM; Proto-oncogene serine/threonine-protein kinase Pim-1; EC2.7.11.1
Gene ID : 5292
mRNA Refseq : NM_002648
Protein Refseq : NP_002639
MIM : 164960
UniProt ID : P11309
Chromosome Location : 6p21.2
Pathway : Acute myeloidleukemia; Jak-STAT signaling pathway; C-MYB transcription factor network;IL-5 Signaling Pathway; Role of Calcineurin-dependent NFAT signaling inlymphocytes; Validated targets of C-MYC transcriptional activation
Function : ATP binding;nucleotide binding; manganese ion binding; metal ion binding; proteinbinding; protein serine/threonine kinase activity; transcription factorbinding

Not For Human Consumption!

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Customer Reviews (3)

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Reviews
01/30/2022

    With the combination of this protein reagent's instructions, I can easily accomplish experimental design and operation.

    04/29/2020

      With consistent and reliable results, this protein reagent is ideal for research projects that require replicability.

      01/02/2020

        Following the use of this protein reagent, the reproducibility of my experiments has significantly improved, and I am highly satisfied.

        Q&As (7)

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        What are the main substrates of PIM1 and how does their interaction affect cellular processes? 01/28/2022

        PIM1 phosphorylates a variety of substrates, including BAD, c-Myc, and eIF4B. Phosphorylation of BAD inhibits its pro-apoptotic function, while phosphorylation of c-Myc and eIF4B enhances their transcriptional and translational activities, promoting cell survival and protein synthesis, respectively.

        How is PIM1 activity regulated by signaling pathways? 05/01/2021

        PIM1 activity is regulated by several signaling pathways, including the PI3K/AKT and JAK/STAT pathways. Activation of these pathways leads to the phosphorylation and activation of PIM1, while inhibition of these pathways can result in reduced PIM1 activity.

        What is the potential of PIM1 as a prognostic marker or therapeutic target in different cancer types? 11/24/2020

        Further research is needed to assess the potential of PIM1 as a prognostic marker and therapeutic target in different cancer types. Understanding the specific roles of PIM1 in various cancers and its interactions with other signaling pathways will help determine its clinical relevance and potential utility in personalized cancer treatment strategies.

        What role does PIM1 play in cancer development? 02/13/2020

        PIM1 has been implicated in promoting cell proliferation, survival, and resistance to apoptosis, contributing to cancer development. Its overexpression has been observed in various cancer types and is associated with poor prognosis. PIM1 also interacts with oncogenic signaling pathways, further promoting tumor growth and progression.

        Are there any potential therapeutic strategies targeting PIM1? 01/23/2018

        Several small molecule inhibitors targeting PIM1 kinase activity have been developed and show promise as potential therapeutics. These inhibitors can selectively inhibit PIM1 activity and have demonstrated anti-tumor effects in preclinical studies. Combination therapy with PIM1 inhibitors and other targeted drugs may also be a viable approach for treating PIM1-driven cancers.

        How does PIM1 regulate cell cycle progression and proliferation? 03/17/2017

        PIM1 regulates cell cycle progression and proliferation by phosphorylating key substrates, such as p27 and CDC25A, which control the activity of cyclin-dependent kinases (CDKs). This phosphorylation promotes the degradation of p27 and stabilizes CDC25A, leading to enhanced CDK activity and cell cycle progression.

        How is PIM1 expression regulated at the transcriptional level? 02/16/2016

        PIM1 expression can be regulated by various factors, including cytokines, growth factors, and oncogenic signals. These factors can activate specific transcription factors, such as STAT3 and NF-κB, which bind to the PIM1 promoter and enhance its transcriptional activity, resulting in increased PIM1 expression.

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