| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
His |
| Description : |
PIM1 is aproto-oncogene which encodes for the serine/threonine kinase of the samename. The PIM1 oncogene was first described in relation to murine T-celllymphomas, as it was the locus most frequently activated by the Moloney murineleukemia virus. Subsequently, the oncogene has been implicated in multiplehuman cancers, including prostate cancer, acute myeloid leukemia and otherhematopoietic malignancies. |
| Form : |
Liquid in 50 mMTris, pH 7.5 + 500 mM NaCl + 0.5 mM EDTA + 0.05% Triton X-100 + 2 mM DTT +50% glycerol. |
| Concentration : |
0.18 mg/mL totalprotein as measured using the Bradford protein assay with BSA as a standard. |
| Molecular Weight : |
40.7 kDa |
| Purity : |
90% as determined bya Coomassie blue-stained SDS-PAGE gel |
| Mass Spectrometry : |
The protein wasdigested with trypsin followed by LC-MS/MS. The resulting MS/MS data verifiedPIM1 identity from 8 peptides that covered 13% of the amino acid sequence ofthe recombinant protein. |
| Assay Conditions : |
The enzyme waspre-diluted in enzyme dilution buffer and assayed in 50 mM Tris (pH 7.5), 10mM MgCl2, 2 mM DTT, 0.025% Triton® X-100, 200 µM ATP, 100 µM PIM1 peptidesubstrate and trace [32P]-γ-ATP for 10 minutes at 30°C. |
| Specific Activity : |
8680 nmole ofphosphate transferred to PIM1 peptide substrate (AKKRRLSA) per minute per mgof total protein at 30°C. Activity determined at a final proteinconcentration of 0.083 µg/mL. |
| Dilution Buffer : |
20 mM Tris (pH 7.5),0.05% Triton® X-100, 0.1 mg/mL BSA, 0.5 mM Na3VO4, 2 mM DTT, 10% Glycerol. |
| Storage Buffer : |
50 mM Tris (pH 7.5),500 mM NaCl, 0.5 mM EDTA, 0.05% Triton® X-100, 2 mM DTT, 50% Glycerol. |
| Storage : |
Store at -80°C. Atfirst use, aliquot and store at -80°C to avoid multiple freeze-thaws. Ifproperly stored at -80°C, this product is stable for 6 months from date ofpurchase. This protein comes out of solution at low salt concentrations.Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
PIM1 |
| Full Length : |
Full L. |
