Revealing the Secret of Our Bacteria Expression Systems (E. coli / Bacillus)
In bacterial expression systems, E. coli is one of the most widely used expression hosts. The techniques for protein expressions in E. coli have been well developed and expanded with the development of biotechnical skills.
Despite of the advances, there are still many tricky problems in the process. Issues such as hard to express and hard to dissolve proteins have limited their applications.
At the request of the industry needs, Creative BioMart gathered a team of experienced scientist staff to address the current state of protein expression in bacteria system and identify obstacles current researchers are facing in order to determine how to best support the field and encourage following success.
With the devotion, many unique techniques have been developed: BacTECTM that perfectly solves the issues of hard-to-express proteins and is capable of achieving soluble expression levels well over 20 g/L, FoldEZTM that designs to deal with hard-to-dissolve proteins and over 98% of the inclusion bodies can be refolded and solubilized with it, OxiCytoTM that expresses proteins having complex disulfide bonds and enable the folding of recombinantly expressed proteins in vivo etc.
The proprietary technologies developed by Creative BioMart properly solved the tricky problems and ensured the smooth conduct of related projects as well as many medium- and small-scale projects to advance the technology and develop the standard practices needed to advance the field.
In addition to this, Creative BioMart has extensive expertise across four expression systems for producing recombinant proteins, monoclonal antibodies, vaccine antigens or disease biomarkers. Whether for structural studies, functional assays, target validation or high throughput screens, we can help.
Protein expression systems allow researchers to look at the wonders of life sciences, biotechnology and medicine.