Principle & Protocol

The purpose of this experimental protocol is to help researchers mastering the basic principles and main steps of prokaryotic expression of the target protein, and master the basic steps and precautions of Coomassie brilliant blue staining and immunoblotting experiment.

Silver staining is an important dyeing method, which is widely used because of its low cost, safe reagents, fast and simple, and high sensitivity. The purpose of this experimental protocol is to help researchers learn the principle, technology and application of Silver Staining.

Coomassie brilliant blue staining method can reach the detection level of microgram level, and the linear dynamic correlation range between staining degree and protein content is wide, which is suitable for quantitative analysis. The purpose of this experimental protocol is to help researchers learn the principle, technology and application of Coomassie brilliant blue dyeing.

Isoelectric focusing electrophoresis usually adopts horizontal plate electrophoresis or tube electrophoresis. It has high resolution and sensitivity, and is particularly suitable for studying the microscopic heterogeneity of proteins. The purpose of this experimental protocol is to help researchers understand the principle, technology and application of Isoelectric Focusing Electrophoresis.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is the most commonly used electrophoretic technique for protein separation. SDS polyacrylamide gel electrophoresis is to add SDS. The purpose of this experimental protocol is to help researchers understand the principle, technology and application of SDS-PAGE.

Polyacrylamide gel is mainly used for protein electrophoresis. Polyacrylamide gel electrophoresis is a process in which polyacrylamide gel is used as a support medium to separate the action of charged particles in an electric field. The purpose of this experimental protocol is to help researchers understand the principle, technology and application of native-PAGE.

The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Kjeldahl method for determining protein concentration. Kjeldahl determination of nitrogen is a commonly used method for analyzing nitrogen content of organic compounds at present. It is one of the most accurate and simple methods for determining total organic nitrogen in samples, and has been taken as the standard inspection method determined internationally and domestically.

BCA method is one of the most commonly used protein concentration detection methods in the world at present. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of BCA method for determining protein concentration, and understand the test method and application of BCA method.

At present, many mature protein concentration determination methods have been developed for different samples. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Lowry method for determining protein concentration, and understand the test method and application of Lowry method.

At present, many mature protein concentration determination methods have been developed for different samples. The purpose of this experimental protocol is to help scientific researchers master the basic principle and main steps of Biuret method for determining protein concentration, and understand the test method and application of Biuret method.