Principle & Protocol
Protocol of the Phosphorylated Antibody Detection Method
This experimental manual is designed to help scientific researchers master the basic principles and main steps of western blot detection of protein phosphorylation level, and understand the application of phosphorylation antibody detection methods.
Principle and Protocol of the PRO-Q Diamond Staining
The purpose of this experiment manual is to help scientific researchers master the basic principles and main steps of Pro-Q Diamond fluorescent dye to detect phosphorylated protein and SYPRO fluorescent dye to detect total protein, and understand the application of Pro-Q Diamond fluorescent dye to detect phosphorylated protein and SYPRO fluorescent dye to detect total protein.
Protocol of the Detection of ³²P Radioactive Markers
The purpose of this lab manual is to help researchers master the basic principles and main steps of 32P radiolabeling detection of phosphorylated proteins and understand the application of 32P radiolabeling detection of phosphorylated proteins method.
Principle and Protocol of Isotope Labeling
The purpose of this experiment guide is to help researchers master the basic principles of iTRAQ technology and understand the experimental process and precautions of iTRAQ.
Principle and Protocol of Fluorescence Labeled Differential Analysis Gel Electrophoresis
The purpose of this experiment manual is to help researchers master the basic principles and main steps of using two-dimensional electrophoresis combined with fluorescent dye labeling to analyze differentially expressed proteins and the method of using DeCyder software to analyze differentially expressed proteins.
Guide of Analysis of Differentially Expressed Proteins by Two-dimensional Electrophoresis
This experimental manual focuses on the main methods of two-dimensional electrophoresis, the analysis methods of differential proteins and the optimization of parameters.
Guide of PCR-assisted Analysis of Protein-DNA Sequence Specificity
The main purpose of this experimental manual is to help scientific researchers understand the principle of PCR-assisted protein-DNA sequence specificity analysis and master the main operating steps and precautions of PCR-assisted protein-DNA sequence specificity analysis.
Principle and Protocol of Yeast One-hybrid Analysis
The purpose of this experiment manual is to help scientific researchers understand the basic principle and application of yeast single hybridization, master the main steps and precautions of yeast single hybridization, learn the production and transformation of yeast receptive state, and the construction and screening of gene library.
Guide of Analysis of Transient Cell Interaction
The purpose of this experiment manual is to help scientific researchers understand the principle of cell transient interaction analysis and master the main operating steps and precautions of cell transient interaction analysis.
Principle and Protocol of ChIP
The purpose of this lab manual is to help researchers master the screening of DNA sequences interacting with target proteins (regulators), to obtain information on protein-DNA interactions, and to master the principles, applications, and major steps of ChIP.