Principle & Protocol
Principle and Protocol of EMSA
The purpose of this laboratory manual is to help researchers understand the principles of electrophoretic mobility analysis techniques and to master the main operational steps and precautions for gel migration.
Guide of Analysis of Promoter Regulatory Elements Based on Plant CARE Prediction Method
The purpose of this experiment manual is to help scientific researchers master the PlantCARE database software of plant cis-acting elements, and analyze the promoter regulatory elements through the PlantCARE online prediction method.
Principle and Protocol of Pull-down Technology
The purpose of this experiment manual is to help scientific researchers learn the experimental principle of pull-down technology to identify protein interaction, and be familiar with the operation process and precautions of MBP pulldown technology.
Guide of Protein Subcellular Co-localization
The purpose of this experiment manual is to help scientific researchers master the preparation of Arabidopsis protoplasts, learn the technology of detecting protein-protein interaction using protein subcellular co-localization method, and be familiar with the operation process and precautions of co-transfection technology.
Principle and Protocol of Co-IP
The laboratory manual is designed to help researchers master the principles of immunoprecipitation and its applications, learn to apply immunoprecipitation methods to detect protein interactions, and become familiar with the operational procedures and precautions for immunoprecipitation techniques.
Principle and Protocol of Yeast Two-hybrid System
The purpose of this experiment manual is to help scientific researchers master the principle and application of the yeast two-hybrid Sos recruited system, learn to use the co-transformation method to screen the interacting proteins and be familiar with the operation process and precautions of the yeast two-hybrid system for library screening.
Principle and Protocol of Yeast Two Hybrid System
The purpose of this experimental protocol is to help researchers to grasp the principle and application of the classic yeast two hybrid system, learn the methods of preparation and transformation of yeast cell competent state, and be familiar with the operation process and experimental precautions of yeast two hybrid system for detecting protein interaction.
Principle and Protocol of Expression and Purification of MBP Fusion Protein
The purpose of this experimental protocol is to help researchers to understand the basic principle of purification of maltose binding protein (MBP) fusion protein. Master the expression and purification methods and main operation steps of MBP fusion protein.
Principle and Protocol of Expression and Purification of His Fusion Protein
The purpose of this experimental protocol is to help researchers to understand the basic principle of fusion protein expression and purification. Master the expression and purification methods of histidine labeled fusion protein, as well as the main operation steps and precautions.
Principle and Protocol of Purification of GST Fusion Protein
The purpose of this experimental protocol is to help researchers to understand the basic principle of fusion protein expression and purification. Master the expression and purification methods of glutathione S-transferase (GST) fusion protein, as well as the main operation steps and precautions.