PARP3
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Official Full Name
poly (ADP-ribose) polymerase family, member 3
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Overview
The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. -
Synonyms
PARP3; poly (ADP-ribose) polymerase family, member 3; ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) like 3 , ADPRTL3; poly [ADP-ribose] polymerase 3; ADPRT3; hPARP 3; IRT1; NAD+ ADP ribosyltransferase 3; pADPRT 3; poly(ADP ribose) polymerase;
- Recombinant Proteins
- Cell & Tissue Lysates
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- Human
- Mouse
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- E.coli
- HEK293
- HEK293T
- Insect Cell
- Insect Cells
- Mammalian Cell
- Sf21 Insect Cell
- Sf9 Insect Cell
- GST
- HA
- His
- His|GST
- Myc
- DDK
- Myc|DDK
- N/A
- Background
- Quality Guarantee
- Case Study
- Involved Pathway
- Protein Function
- Interacting Protein
- PARP3 Related Research Area
What is PARP3 protein?
PARP3 is an enzyme that belongs to the poly (ADP-ribose) polymerase (PARP) family. PARP3, also known as ADP-ribosyltransferase diphtheria toxin-like 3 (ARTD3), is involved in post-translational modification of proteins through the addition of ADP-ribose groups to target proteins. It plays a role in several cellular processes, including DNA repair, replication, and transcription regulation.
What is the function of PARP3 protein?
The main function of PARP3 is to facilitate DNA repair by recognizing damaged DNA and recruiting other repair proteins to the site of damage. It is primarily involved in base excision repair (BER), which is responsible for repairing DNA damage caused by oxidative stress and alkylating agents. PARP3 binds to DNA strand breaks and synthesizes ADP-ribose polymers, which then recruit DNA repair factors to the site of damage.
PARP3 related signaling pathway
The signaling pathway of PARP3 involves its activation in response to DNA damage. PARP3 is recruited to sites of DNA damage, where it binds to and is activated by single-stranded DNA breaks. Upon activation, PARP3 catalyzes the PARylation of target proteins, which can modulate various DNA repair pathways, such as base excision repair and homologous recombination.
PARP3 has also been implicated in other cellular processes, including transcriptional regulation, alternative splicing, and telomere maintenance. It interacts with other proteins, such as DNA repair factors and chromatin remodeling complexes, to exert its functions in these pathways.
PARP3 Related Diseases
In terms of PARP3-related diseases, alterations in PARP3 activity or expression have been implicated in various pathological conditions, including cancer, neurodegenerative disorders, immune dysfunction, and cardiovascular diseases. Dysregulation of PARP3 can result in impaired DNA repair mechanisms and genomic instability, leading to the development and progression of cancer. Additionally, PARP3 dysfunction has been associated with neurodegenerative diseases like Alzheimer's and Parkinson's disease, where aberrant DNA repair processes contribute to neuronal damage and apoptosis.
Biomedical Application of PARP3 Protein
- DNA Repair: PARP3 is involved in the repair of DNA damage, particularly in the base excision repair (BER) pathway. It interacts with other DNA repair proteins, such as XRCC1, and helps in the restoration of damaged DNA. Understanding the mechanisms of PARP3-mediated DNA repair can lead to the development of strategies to enhance DNA repair processes in diseases related to DNA damage.
- Cancer Therapy: PARP inhibitors, which target PARP proteins, have shown promise in cancer treatment, particularly in tumors with deficiencies in DNA repair pathways, such as BRCA-mutated cancers. PARP3 is being studied as a potential target for PARP inhibitor therapy. Inhibition of PARP3 in cancer cells can result in synthetic lethality, where cancer cells with DNA repair defects cannot survive. Clinical trials are underway to evaluate the efficacy of PARP inhibitors, including those targeting PARP3, in various types of cancers.
High Purity
Fig1. SDS-PAGE (PARP3-2607H)
(Ryan M. Centko, 2020)
Fig3. Dose response curves for inhibition of PARP isoforms 1, 3, and 16 by latonduine A (1), 5, and 6 using a compound concentration gradient between 1 nM and 1 mM. (A) Latonduine A (1); (B) compound 5; and (C) compound 6.
(Ali H Hassan, 2016)
Fig4. Mitotic phenotypes resulting from depletion or inhibition of PARPs or PARG. Schematics of the phenotypes observed in (A) metaphase and (B) anaphase, and the corresponding microscopy pictures are shown. Chromosomes are shown in blue. On the example of a multipolar spindle, tubulin is shown in red. Telomere fusion upon PARP3 depletion is visualized by FISH using PNA probe for telomeres (red); the microscopy pictures were kindly provided by Françoise Dantzer. Ultrafine anaphase DNA bridges in PARP1-depleted or inhibited cells are visualized with anti-PICH staining in green; the microscopy pictures were kindly provided by Simon Gemble and Mounira Amor- Guéret.
(Anastasiya A. Kosova, 2019)
Fig4. Demonstration of primer MARylation in Mmu WCE (A) and in the system reconstituted from recombinant proteins (B). (A) Mmu cell extract proteins (0.5 mg/mL) were incubated for 5 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl2 and 0.5 mM NAD+ in the absence or presence of PARG and olaparib. (B) Recombinant proteins were incubated for 10 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl2, 0.1 mM dNTPs, and 0.5 mM NAD+ (when indicated). Lanes 1 in A and B correspond to the initial primer (control).
PARP3 involved in several pathways and played different roles in them. We selected most pathways PARP3 participated on our site, such as Base excision repair, which may be useful for your reference. Also, other proteins which involved in the same pathway with PARP3 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Base excision repair | POLD1;PNKP;MBD4;POLE4;PCNA;nth;NEIL2;PARP3;POLE2 |
PARP3 has several biochemical functions, for example, NAD+ ADP-ribosyltransferase activity, catalytic activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PARP3 itself. We selected most functions PARP3 had, and list some proteins which have the same functions with PARP3. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| NAD+ ADP-ribosyltransferase activity | PARP12A;GIG2J;PARP16;SIRT6;PARP3;SIRT4;PARP12;ART1;GIG2F |
| catalytic activity | ZRANB3;SYN2;CKBB;CPS1;GTF2F2A;PBLD2;MOSC2;ASAH1;ECI2 |
PARP3 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PARP3 here. Most of them are supplied by our site. Hope this information will be useful for your research of PARP3.
MTHFD2; RPA3; HIST2H2BE; HIST2H3D
- Q&As
- Reviews
Q&As (5)
Ask a questionPARP3 interacts with various proteins involved in DNA repair, forming complexes that coordinate the repair process.
Dysregulation of PARP3 can lead to genomic instability and impaired DNA repair, contributing to the development and progression of certain cancers.
While there are research tools for studying PARP3 activity in the laboratory, clinical diagnostic tools targeting PARP3 are still in the early stages of development.
Emerging research suggests that PARP3 may play a role in immune regulation, and its modulation could potentially impact immune responses in diseases such as cancer.
Some clinical trials are exploring the efficacy and safety of PARP inhibitors, and their potential application in cancers where PARP3 is implicated.
Customer Reviews (3)
Write a reviewAn additional advantage of selecting the PARP3 protein is the outstanding technical support offered by its manufacturer.
Their unwavering commitment to customer satisfaction assures me that any challenges or queries I may encounter will be expertly resolved.
The combination of the protein's excellence and the manufacturer's exceptional technical support equips me with the necessary resources to overcome experimental obstacles and achieve groundbreaking results.
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