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PIN1

  • Official Full Name

    peptidylprolyl cis/trans isomerase, NIMA-interacting 1

  • Overview

    The enzyme Peptidylprolyl isomerase (Pin1) is responsible for flipping the proline ring from the cis to trans conformation. This enzyme regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Pin1 is concentra
  • Synonyms

    PIN1; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; protein (peptidyl prolyl cis/trans isomerase) NIMA interacting 1; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; dod; PPIase Pin1; rotamase Pin1; protein (peptidyl-prolyl cis/trans iso;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • Mammalian Cell
  • Mammalian cells
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • Myc
  • DDK
  • N/A
  • N
  • T7
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human PIN1-351H Recombinant Human Peptidylprolyl Cis/trans Isomerase, NIMA-interacting 1 E.coli N/A
Human PIN1-3825H Recombinant Human PIN1, His tagged E.coli His Met 1-Glu 163
Human PIN1-30964TH Recombinant Human PIN1 E.coli N/A
Human PIN1-29590TH Recombinant Human PIN1, His-tagged E.coli His
Human PIN1-1722H Recombinant Human PIN1, GST-tagged E.coli GST 1-163
Human PIN1-3826H Recombinant Human PIN1 protein E.coli N/A Met 1-Glu 163
Human PIN1-2901H Recombinant Human PIN1, T7-tagged E.coli T7
Human PIN1-3179HCL Recombinant Human PIN1 293 Cell Lysate HEK293 N/A
Human PIN1-4907H Recombinant Human PIN1 Protein (Met1-Glu163), N-GST tagged E.coli N-GST Met1-Glu163
Human PIN1-2569H Active Recombinant Human PIN1 protein E.coli N/A Met1-Glu163
Human PIN1-1058HFL Recombinant Full Length Human PIN1 Protein, C-Flag-tagged Mammalian cells Flag
Human PIN1-6628H Recombinant Human PIN1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human PIN1-1682H Recombinant Human PIN1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human PIN1-1682H-B Recombinant Human PIN1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Pin1-1167M Recombinant Mouse Pin1 protein, His-tagged E.coli His Ala2~Glu165 (Accession # Q9QUR7)
Mouse PIN1-12817M Recombinant Mouse PIN1 Protein Mammalian Cell His
Mouse PIN1-6747M-B Recombinant Mouse PIN1 Protein Pre-coupled Magnetic Beads HEK293
Mouse PIN1-6747M Recombinant Mouse PIN1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Pin1-4871M Recombinant Mouse Pin1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Cynomolgus Monkey PIN1-791C Recombinant Cynomolgus PIN1 Protein, His-tagged Mammalian Cell His
Cynomolgus Monkey PIN1-534C Recombinant Cynomolgus Monkey PIN1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Cynomolgus Monkey PIN1-534C-B Recombinant Cynomolgus Monkey PIN1 Protein Pre-coupled Magnetic Beads HEK293
Zebrafish PIN1-11011Z Recombinant Zebrafish PIN1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • PIN1 Related Articles

What is PIN1 protein?

PIN1 (peptidylprolyl cis/trans isomerase, NIMA-interacting 1) gene is a protein coding gene which situated on the short arm of chromosome 19 at locus 19p13. The protein encoded by this gene is human PIN1 , a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. PIN1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of PIN1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. The PIN1 protein is consisted of 163 amino acids and its molecular mass is approximately 18.2 kDa.

What is the function of PIN1 protein?

The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival.

PIN1 Related Signaling Pathway

The signaling pathway involved in PIN1 protein is mainly involved in regulating protein phosphorylation and conformational change, thus affecting cell signal transduction and regulating a variety of biological processes, including NMP signaling pathway, cell growth factor signaling pathway, Wnt/β-catenin signaling pathway, etc. For example, PIN1 is involved in the regulation of cell growth, survival and metabolism by regulating the phosphorylation of Akt and mTOR and the stability of transcription factors.

PIN1 Related Diseases

The abnormal function of PIN1 protein is closely related to the occurrence and development of many diseases, including cancer, nervous system diseases, cardiovascular diseases and so on. PIN1 is also associated with a number of other diseases, such as diabetes, liver disease, kidney disease, etc.

PIN1-7.jpg

Fig1. Accumulation of NFTs and Aβ is one of the pathogenic factors of AD. NFTs and Aβ are products of Tau and APP processing, respectively.

Bioapplications of PIN1

At present, the biological application of PIN1 protein is mainly in the field of cancer therapy. The high expression of PIN1 in cancer cells is associated with the enhancement of drug resistance, proliferation and invasion ability. Therefore, the development of anti-cancer drugs targeting PIN1 has become a hot research direction.

High Purity

High Bioactivity

PIN1-2.jpg

Fig2. Activity Data. (PIN1-2569H)

Case Study 1: Juan Long, 2024

PIN1-3.jpg

Fig1. Western blot analysis for LC3 in chloroquine (CQ) treated U2OS cells with stable expression of tagged Pin1 or Pin1W34A.

Case Study 2: Qiaoxia Zhou, 2021

PIN1-4.jpg

Fig2. Immunofluorescence microscopy shows the common position of Pin1 and HBx in both cytoplasm and nucleus.

PIN1-5.jpg

Fig1. Pin1-FoxO3 axis might be specific for cancer cells to accommodate the requirements of autophagy. (Juan Long, 2024)

PIN1-6.jpg

Fig2. In HPV-infected cells, overexpression of Pin1 causes NF-κB nuclear retention and activation of STAT3. (Jingyi Li, 2021)

PIN1 involved in several pathways and played different roles in them. We selected most pathways PIN1 participated on our site, such as RIG-I-like receptor signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with PIN1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
RIG-I-like receptor signaling pathwayIKBKE;IFNA10;MAP3K7;IFNW1;TRIM25;IL12A;IFNE;DAK;CASP10

PIN1 has several biochemical functions, for example, GTPase activating protein binding, mitogen-activated protein kinase kinase binding, peptidyl-prolyl cis-trans isomerase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PIN1 itself. We selected most functions PIN1 had, and list some proteins which have the same functions with PIN1. You can find most of the proteins on our site.

Function Related Protein
GTPase activating protein bindingTUBB;GNAI1;PLCD1;TSC1;PIN1;GNAI3;PTBP3;MEX3B;RGS14
mitogen-activated protein kinase kinase bindingTRIB1;BRAF;TRIB3;IGBP1;PIN1;MAPK8IP3;ARRB1;TAOK2;MAP3K11
peptidyl-prolyl cis-trans isomerase activityFKBP1AA;PPIFA;FKBP9;CWC27;PPIAL4C;FKBP1AB;PPP2R4;FKBP11;PPIF
phosphoserine bindingPIN1;YWHAB;NEDD4;YWHAE
phosphothreonine bindingNEDD4;PIN1
protein bindingABCG2;FAM127B;CDC26;VTA1;STMN2;CIRH1A;AZU1;RFC3;PKP1

PIN1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PIN1 here. Most of them are supplied by our site. Hope this information will be useful for your research of PIN1.

TP53; ARHGAP8

Yun, HJ; Kim, JY; et al. Prolyl-isomerase Pin1 Impairs Trastuzumab Sensitivity by Up-regulating Fatty Acid Synthase Expression. ANTICANCER RESEARCH 34:1409-1416(2014).
Driver, JA; Zhou, XZ; et al. Regulation of Protein Conformation by Pin1 Offers Novel Disease Mechanisms and Therapeutic Approaches in Alzheimer's Disease. DISCOVERY MEDICINE 92:93-99(2014).
  • Q&As
  • Reviews

Q&As (7)

Ask a question
Are there therapeutic strategies targeting PIN1 for disease intervention? 01/22/2023

Yes, studies have explored the therapeutic potential of targeting PIN1 activity. Compounds inhibiting PIN1-mediated protein isomerization have shown promise as anticancer agents in preclinical studies. However, further research is needed to develop safe and effective therapeutic interventions.

How is the protein PIN1 involved in cancer development? 02/25/2021

An aberrant expression of PIN1 has been observed in various types of cancer. It has been found to contribute to cancer development by promoting cell proliferation, inhibiting apoptosis, and facilitating tumor angiogenesis. Additionally, PIN1 has been implicated in regulating the activity of oncogenes and tumor suppressor genes, further highlighting its involvement in cancer progression.

How does the protein PIN1 contribute to cellular processes? 08/05/2019

The protein PIN1 is a peptidyl-prolyl cis/trans isomerase that plays a critical role in regulating the conformational changes of phosphorylated proteins, particularly those involved in cell cycle progression, DNA damage response, and gene transcription. It mediates the isomerization of proline residues, thereby impacting protein stability, protein-protein interactions, and subcellular localization, influencing various cellular processes.

What molecular mechanisms contribute to the aberrant expression of PIN1 in various types of cancer? 06/09/2019

The abnormal expression of PIN1 in cancer can be attributed to several molecular mechanisms. One mechanism is the dysregulation of PIN1 transcription, often caused by genetic alterations or epigenetic modifications. Another mechanism involves the dysregulation of post-translational modifications that affect PIN1 protein stability and activity. Furthermore, upstream signaling pathways, such as growth factor signaling, can activate PIN1 expression. Additionally, oncogenic transcription factors and signaling cascades can directly or indirectly enhance PIN1 expression in cancer cells.

How does PIN1 regulate protein conformation? 05/27/2018

PIN1 exerts its regulatory function through the isomerization of proline residues that precede phosphorylated serine or threonine residues. This conformational change alters the stability, interactions, and localization of proteins, consequently influencing crucial cellular processes.

How does PIN1 regulate the activity of oncogenes and tumor suppressor genes in cancer? 03/04/2018

PIN1 interacts with oncogenes and tumor suppressor genes at various levels to modulate their activity. PIN1 mainly exerts its effect through protein isomerization, which can induce conformational changes in target proteins. PIN1 can stabilize oncogenic proteins or promote their degradation, thus affecting their activity. Similarly, PIN1 can modify the conformation of tumor suppressor proteins, leading to altered protein interactions and functional outcomes. By regulating the activity of these key players, PIN1 can influence the overall balance between oncogenic and tumor-suppressive signaling pathways in cancer cells.

What are the consequences of PIN1 dysregulation? 02/18/2017

Dysregulation of PIN1 has been associated with various diseases, including cancer, neurodegenerative disorders, and aging-related pathologies. Aberrant PIN1 activity can disrupt cellular signaling pathways, contribute to uncontrolled cell growth, and impair protein quality control mechanisms, leading to pathological conditions.

Customer Reviews (3)

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Reviews
09/21/2019

    Following the implementation of this protein reagent, the reproducibility of my experiments has significantly improved, leaving me highly satisfied.

    09/26/2017

      Approaching my research results with absolute confidence, this experimental reagent's high quality elevates my research expertise.

      02/25/2017

        The dilution effect of the reagent is excellent, providing a wide measurement range.

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