SMAD3

  • Official Full Name

    SMAD family member 3

  • Background

    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
  • Synonyms

    SMAD3; SMAD family member 3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3;
    • Type:
    • Recombinant Proteins
    • Protein Pre-coupled Magnetic Beads
    • Cell & Tissue Lysates
    • Species :
    • Chicken
    • Human
    • Human/Mouse/Rat
    • Mouse
    • Rat
    • Rhesus Macaque
    • Source :
    • E.coli
    • HEK293
    • HEK293T
    • Human
    • Insect Cell
    • Insect Cells
    • Mammalian Cell
    • Mammalian cells
    • Tag :
    • Flag
    • GST
    • His
    • His (Fc)
    • Avi
    • His|MBP
    • SUMO
    • Myc
    • DDK
    • Myc|DDK
    • N/A
    Species Cat.# Product name Source (Host) Tag Price
    Human SMAD3-1094H Recombinant Human SMAD Family Member 3, GST-tagged E.coli GST
    Human SMAD3-4599H Recombinant SMAD Family Member 3, His-tagged Human His
    Human SMAD3-327H Recombinant Human SMAD3 protein, His/MBP-tagged E.coli His/MBP
    Human SMAD3-6915H Recombinant Human SMAD Family Member 3, His-tagged E.coli His
    Human SMAD3-31191TH Recombinant Human SMAD3 E.coli N/A
    Human SMAD3-31190TH Recombinant Human SMAD3, His-tagged E.coli His
    Human SMAD3-295H Recombinant Human SMAD3 protein, GST-tagged E.coli GST
    Human SMAD3-326H Recombinant Human SMAD3 protein, His/MBP-tagged E.coli His/MBP
    Human SMAD3-4788H Recombinant Human SMAD3 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
    Human SMAD3-304H Recombinant Human SMAD3 Protein, His-tagged E.coli His
    Human SMAD3-1918H Recombinant Human SMAD3 Protein, MYC/DDK-tagged HEK293 Myc/DDK
    Human SMAD3-227HFL Active Recombinant Full Length Human SMAD3 Protein, C-Flag-tagged Mammalian cells Flag
    Human SMAD3-2256H Recombinant Human SMAD3 protein, His-tagged Insect Cell His
    Human SMAD3-2041H-B Recombinant Human SMAD3 Protein Pre-coupled Magnetic Beads HEK293
    Human SMAD3-3506H Recombinant Human SMAD3 protein, His-SUMO-tagged E.coli His-SUMO
    Human SMAD3-2787H Recombinant Human SMAD3 Protein (Asp220-Ser425), His tagged E.coli His
    Human SMAD3-1973HF Active Recombinant Full Length Human SMAD3 Protein, GST-tagged E.coli GST
    Human SMAD3-2041H Recombinant Human SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
    Mouse SMAD3-532M Recombinant Mouse Smad3, His-GST tagged Insect Cell GST
    Mouse SMAD3-001MCL Recombinant Mouse SMAD3 cell lysate Insect Cell N/A
    Mouse Smad3-5951M Recombinant Mouse Smad3 Protein, Myc/DDK-tagged HEK293T Myc/DDK
    Mouse Smad3-305M Recombinant Mouse Smad3 Protein, His-tagged E.coli His
    Rat SMAD3-5608R Recombinant Rat SMAD3 Protein Mammalian Cell His
    Rat SMAD3-5267R Recombinant Rat SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
    Rat SMAD3-5267R-B Recombinant Rat SMAD3 Protein Pre-coupled Magnetic Beads HEK293
    Human/Mouse/Rat Smad3-0256H Recombinant Human/Mouse/Rat Smad3 protein, His&GST-tagged Insect Cells His/GST
    Rhesus Macaque SMAD3-4331R Recombinant Rhesus monkey SMAD3 Protein, His-tagged Mammalian Cell His
    Rhesus Macaque SMAD3-4147R Recombinant Rhesus Macaque SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
    Rhesus Macaque SMAD3-4147R-B Recombinant Rhesus Macaque SMAD3 Protein Pre-coupled Magnetic Beads HEK293
    Chicken SMAD3-6045C Recombinant Chicken SMAD3 Mammalian Cell His
    • Involved Pathway
    • Protein Function
    • Interacting Protein
    • SMAD3 Related Articles
    • SMAD3 Related Gene Family
    • SMAD3 Related Research Area
    • SMAD3 Related Signal Pathway

    SMAD3 involved in several pathways and played different roles in them. We selected most pathways SMAD3 participated on our site, such as FoxO signaling pathway, Cell cycle, Endocytosis, which may be useful for your reference. Also, other proteins which involved in the same pathway with SMAD3 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

    Pathway Name Pathway Related Protein
    FoxO signaling pathwayNLK;KLF2B;RAF1A;MAPK3;PLK3;FOXO6;PIK3R3A;FOXO3B;G6PCA.1
    Cell cycleCEP290;MCM4;MSH5;KIF20A;ANAPC11;PHF8;HAUS2;MCM7;WEE1
    EndocytosisSMAD3A;FGFR3;GBF1;RLN1;IST1;ARFGAP2;PARD6GA;SPG20;VTA1
    Wnt signaling pathwayPPP3R2;FZD8;PSEN1;RHOAD;MMP7;PORCNL;BAMBIA;PRKCA;DVL3A
    TGF-beta signaling pathwayTGFBR1B;GDF7;BMP6;BMPR1BA;INHBE;SKP1;SP1;SMAD4;ACVR1C
    Hippo signaling pathwayLEF1;FZD2;FZD7;MOB1A;BTRC;LLGL2;TP73;CDH1;TEAD2
    Adherens junctionMET;ACTB1;FGFR1A;RAC3A;PTPRJ;SSX2IPA;SNAI2;Fert2;PTPRF
    Signaling pathways regulating pluripotency of stem cellsPIK3CB;PCGF2;PIK3CD;FZD1;FZD5;KRAS;PIK3CG;HNF1A;SMAD3
    Chagas disease (American trypanosomiasis)TGFBR2;SMAD2;NFKB1;MAPK1;IKBKG;C1QB;IL1B;TICAM1;PIK3CD
    Hepatitis BDDB2;HSPG2;CREB3L1;MAVS;IKBKB;CREB3L3;BIRC5;PCNA;IFNB1
    HTLV-I infectionELK4;ANAPC7;FZD10;IKBKB;PIK3R2;POLE3;Fzd4;MSX1;ANAPC11
    Pathways in cancerGNG8;CXCR4;GNG7;FZD6;FZD5;PTCH2;TCF7L2;ARHGEF1;TFG
    Colorectal cancerCASP9;MAPK10;MAPK1;RAC1;BAD;TGFB2;BRAF;TGFB1;MSH2
    Pancreatic cancerRB1;PIK3R1;MAP2K1;CDC42;MAPK1;TGFB3;PIK3R3;CASP9;AKT1
    Chronic myeloid leukemiaGRB2;SHC4;RB1;PIK3CD;PIK3R3;TGFBR2;CTBP1;SHC1;NFKBIA
    Inflammatory bowel disease (IBD)HLA-DRB5;STAT4;TLR5;IFNGR1;IL13;FOXP3;TGFB3;NFKB1;IFNGR2

    SMAD3 has several biochemical functions, for example, R-SMAD binding, RNA polymerase II activating transcription factor binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SMAD3 itself. We selected most functions SMAD3 had, and list some proteins which have the same functions with SMAD3. You can find most of the proteins on our site.

    Function Related Protein
    R-SMAD bindingSMAD4;ZC3H3;PARP1;PMEPA1;TRIM33;C18orf1;MEN1;PAX6;ZEB2
    RNA polymerase II activating transcription factor bindingSMAD3;LMO2;HIPK2;SIN3A;TBX6;RB1;HEY2;POU1F1;SETD3
    RNA polymerase II core promoter proximal region sequence-specific DNA bindingELF4;ZIC3;MIXL1;MLXIPL;HOXA2;NR2C1;Ar;HLTF;MYOG
    bHLH transcription factor bindingTCF3B;USF1;ISL1;TCF3;TCF3A;SOX9;USF2;TCF12;RUNX2
    beta-catenin bindingPTBP3;CTNNA3;SOX17;DVL3;SHROOM2;VCL;CDH24;LZIC;SMAD7
    chromatin DNA bindingPER1;VAX2;APEX1;WBP2;THRA;SMAD3;PRDM14;GRHL2;GRHL1
    co-SMAD bindingTRIM33;GATA4;SMAD3;USP9X;FOXH1;SMAD2;TGIF1;SMAD1;CITED1
    collagen bindingCCBE1;PDGFB;CTSS;TLL1;CTSL1;TGFBI;CTSB;LRRC15;SMAD7
    core promoter proximal region sequence-specific DNA bindingSP3;MEF2C;UHRF1;DMRT3A;DMRTC2;SMAD4;ZFP516;DMRT3;GATA3
    enhancer bindingSOX9;TIPARP;TCF12;SMAD3;MESP1;LEF1;SP1;RAI1;SMAD2
    identical protein bindingDAPK1;AMOTL2;SERPINA1A;TYROBP;PLN;FNBP1;VPS4B;SH3GL2;APOH
    phosphatase bindingPPP1R26;PPP1R36;SH2D4A;KIAA0649;ELFN2;CRY2;CRY1;SPRED1;MAPK1
    protein bindingEDNRB;ASAP3;ERP27;USP36;O3far1;FCN3;C12orf68;ZNF614;GNA14
    protein heterodimerization activitySMC1A;RRAGD;SNX6;BCL2L1;HIST1H2B5;HIST1H4E;ADIPOR2;H2-AA;CREB3L3
    protein homodimerization activityADAM10;UGT1A6;CRYBA1;GLA;WWTR1;GRIK2;GRM6;SLC11A1;FAM109B
    protein kinase bindingNIPAL1;AKAP1;ADCY6;CCND3;PPP1CB;TNIP2;CCNA2;MAP2K3;TPRKB
    sequence-specific DNA bindingUNCX;MAF;RERE;JUNBB;NR2E1;LHX5;FOXS1;FOXI2;NKX2-4
    transcription factor activity, RNA polymerase II core promoter sequence-specificNKX3;MAX;TFAP2B;HEY2;MEF2C;HEYL;SMAD3;PAX7;HEY1
    contributes_to transcription factor activity, protein bindingSMAD3;SMAD4
    transcription factor activity, sequence-specific DNA bindingFLI1;STAT2;TTC4;ZRANB2;ZNF726;GATA6;FOXN2B;ZBTB17;TFAM
    contributes_to transcription factor activity, sequence-specific DNA bindingVDRA;VDRB;NKX2;SMAD4;HNRNPAB;NFE2L2A;TAF12;SOX1A;TAF10
    transcription factor bindingTAF1;NAB1;APBB1;PSMD10;HMGA1;HES3;CREBBP;LCOR;SIRT1
    transcription regulatory region DNA bindingZFHX3;CALCOCO1;GFI1;RFX3;TAL1;PAX8;GLIS2;FOXC1;ATMIN
    transforming growth factor beta receptor bindingSMURF2;GDF15;FKBP1A;INHBA;BMP15;GDF9;BMP7A;INHBB;LEFTY2
    transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activitySMAD3;SMAD1;SMAD9;SMAD2
    ubiquitin bindingOTUB1A;USP5;KCNS1;IDE;FAF1;MARK4;SQSTM1;UBXN2B;TRIM32
    ubiquitin protein ligase bindingUBE2R2;ANAPC2;HSPA1L;CXCR4;PRKAR2B;CUL9;ASB4;RPA2;TMEM173
    zinc ion bindingBTR12;LNPEP;ZNF385B;RARG;ZFYVE1;GATA2;ADAM7;MMP23BB;RTN4IP1

    SMAD3 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SMAD3 here. Most of them are supplied by our site. Hope this information will be useful for your research of SMAD3.

    SMAD4; SKI

    Smithrithee, R; Niyonsaba, F; et al. Human beta-defensin-3 increases the expression of interleukin-37 through CCR6 in human keratinocytes. JOURNAL OF DERMATOLOGICAL SCIENCE 77:46-53(2015).
    McMillin, M; Galindo, C; et al. Gli1 activation and protection against hepatic encephalopathy is suppressed by circulating transforming growth factor beta 1 in mice. JOURNAL OF HEPATOLOGY 61:1260-1266(2014).
    TGF
    • Q&As
    • Reviews

    Q&As (6)

    Ask a question
    What is the method used to determine the expression level and activity of SMAD3 protein? 12/05/2022

    The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

    How are the interactions between SMAD3 protein and other proteins regulated? 05/25/2022

    interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

    How is SMAD3 protein involved in the TGF-β signaling pathway? 04/28/2022

    In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

    How is the expression level of SMAD3 protein regulated? 03/05/2022

    expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

    What is the main function of SMAD3 protein? 01/25/2022

    The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

    What is SMAD3 protein? 07/22/2020

    SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.

    Customer Reviews (3)

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    Reviews
    05/14/2021

      High expression level, good stability, suitable for high precision experiments. -

      03/08/2021

        Compared with other brands I have used, this brand is more stable and reliable. -

        01/29/2021

          Strong antibacterial activity, not easy to be contaminated. -

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