Recombinant Mouse SMAD3 cell lysate

• Cat.No.: SMAD3-001MCL
• Product Overview: Mouse SMAD3 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Smad3 MAD homolog 3 (Drosophila) [ Mus musculus ]
• Official Symbol: SMAD3
• Synonyms: SMAD3; MAD homolog 3 (Drosophila); mothers against decapentaplegic homolog 3; mad3; mMad3; Smad 3; SMAD family member 3; mothers against DPP homolog 3; Madh3; AU022421
• Gene ID: 17127
• mRNA Refseq: NM_016769
• Protein Refseq: NP_058049
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adipogenesis, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: DNA binding; R-SMAD binding; RNA polymerase II activating transcription factor binding; SMAD binding; beta-catenin binding; chromatin DNA binding; chromatin binding; co-SMAD binding; collagen binding; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; metal ion binding; phosphatase binding; protein binding; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding

Reviews

05/14/2021

High expression level, good stability, suitable for high precision experiments.

03/08/2021

Compared with other brands I have used, this brand is more stable and reliable.

01/29/2021

Strong antibacterial activity, not easy to be contaminated.

Q&As

What is the method used to determine the expression level and activity of SMAD3 protein?

The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

How are the interactions between SMAD3 protein and other proteins regulated?

interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

How is SMAD3 protein involved in the TGF-β signaling pathway?

In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

How is the expression level of SMAD3 protein regulated?

expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

What is the main function of SMAD3 protein?

The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

What is SMAD3 protein?

SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.