Active Recombinant Full Length Human SMAD3 Protein, GST-tagged

• Cat.No.: SMAD3-1973HF
• Product Overview: Full-length human Smad3 [1-425(end) amino acids of accession number NP_005893.1] was expressed as N-terminal GST-fusion protein (75 kDa) using E.coli expression system. GST-Smad3 was purified by using glutathione sepharose chromatography

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The phosphorylational ability of the protein was determined by ELISA. The substrate protein was incubated with the indicated enzyme, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
• Protein length: 1-425(end)
• Purity: 61%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: SMAD family member 3
• Official Symbol: SMAD3
• Synonyms: MADH3; JV15-2; HsT17436
• Protein Refseq: NP_005893.1
• UniProt ID: Q9GKR4

Reviews

05/14/2021

High expression level, good stability, suitable for high precision experiments.

03/08/2021

Compared with other brands I have used, this brand is more stable and reliable.

01/29/2021

Strong antibacterial activity, not easy to be contaminated.

Q&As

What is the method used to determine the expression level and activity of SMAD3 protein?

The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

How are the interactions between SMAD3 protein and other proteins regulated?

interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

How is SMAD3 protein involved in the TGF-β signaling pathway?

In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

How is the expression level of SMAD3 protein regulated?

expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

What is the main function of SMAD3 protein?

The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

What is SMAD3 protein?

SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.