Active Recombinant Full Length Human SMAD3 Protein, GST-tagged

Cat.No. : SMAD3-1973HF
Product Overview : Full-length human Smad3 [1-425(end) amino acids of accession number NP_005893.1] was expressed as N-terminal GST-fusion protein (75 kDa) using E.coli expression system. GST-Smad3 was purified by using glutathione sepharose chromatography
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Source : E. coli
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The phosphorylational ability of the protein was determined by ELISA. The substrate protein was incubated with the indicated enzyme, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
Protein length : 1-425(end)
Purity : 61%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
Gene Name : SMAD family member 3
Official Symbol : SMAD3
Synonyms : MADH3; JV15-2; HsT17436
Protein Refseq : NP_005893.1
UniProt ID : Q9GKR4

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the method used to determine the expression level and activity of SMAD3 protein? 12/05/2022

The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

How are the interactions between SMAD3 protein and other proteins regulated? 05/25/2022

interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

How is SMAD3 protein involved in the TGF-β signaling pathway? 04/28/2022

In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

How is the expression level of SMAD3 protein regulated? 03/05/2022

expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

What is the main function of SMAD3 protein? 01/25/2022

The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

What is SMAD3 protein? 07/22/2020

SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.

Customer Reviews (3)

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Reviews
05/14/2021

    High expression level, good stability, suitable for high precision experiments.

    03/08/2021

      Compared with other brands I have used, this brand is more stable and reliable.

      01/29/2021

        Strong antibacterial activity, not easy to be contaminated.

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