Recombinant Human SMAD3 protein, GST-tagged

• Cat.No.: SMAD3-295H
• Product Overview: Recombinant Human SMAD3(C-terminal SXS deletion) fused with GST tag at N-terminal was expressed in E. coli.

Specification

• Description: SMAD3 is a direct mediator of transcriptional activation by the TGF receptor. The activity of SMAD3 is regulated by the TGF receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGF stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor. SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGF response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: 84 kDa
• Purity: > 85%
• Applications: Western Blot
• Stability: 1 year at -70 centigrade from the date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: SMAD3 SMAD family member 3 [ Homo sapiens ]
• Official Symbol: SMAD3
• Synonyms: SMAD3; SMAD family member 3; MAD, mothers against decapentaplegic homolog 3 (Drosophila) , MADH3, SMAD, mothers against DPP homolog 3 (Drosophila); mothers against decapentaplegic homolog 3; HsT17436; JV15 2; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; LDS1C; MADH3; JV15-2; HSPC193; MGC60396; DKFZp586N0721; DKFZp686J10186
• Gene ID: 4088
• mRNA Refseq: NM_005902
• Protein Refseq: NP_005893
• MIM: 603109
• UniProt ID: P84022
• Chromosome Location: 15q21-q22
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adipogenesis, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: R-SMAD binding; RNA polymerase II activating transcription factor binding; beta-catenin binding; chromatin DNA binding; co-SMAD binding; collagen binding; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; metal ion binding; phosphatase binding; protein binding; contributes_to protein binding transcription factor activity; protein homodimerization activity