Recombinant Human/Mouse/Rat Smad3 protein, His&GST-tagged

• Cat.No.: Smad3-0256H
• Product Overview: Recombinant Human/Mouse/Rat Smad3 protein(NP_058049.3)(Met1-Ser425), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human/Mouse/Rat
• Tag: His&GST
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM GSH, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant human / mouse / rat SMAD3 consists of 662 amino acids and has a calculated molecular mass of 75.9 kDa.
• Proteinlength: Met1-Ser425
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

05/14/2021

High expression level, good stability, suitable for high precision experiments.

03/08/2021

Compared with other brands I have used, this brand is more stable and reliable.

01/29/2021

Strong antibacterial activity, not easy to be contaminated.

Q&As

What is the method used to determine the expression level and activity of SMAD3 protein?

The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

How are the interactions between SMAD3 protein and other proteins regulated?

interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

How is SMAD3 protein involved in the TGF-β signaling pathway?

In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

How is the expression level of SMAD3 protein regulated?

expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

What is the main function of SMAD3 protein?

The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

What is SMAD3 protein?

SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.