Recombinant Mouse Smad3 Protein, His-tagged
Cat.No. : | Smad3-305M |
Product Overview : | Recombinant Mouse Smad3(Phe231~Ser425) fused with His tag at N-terminal was expressed in E. coli. |
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Source : | E. coli |
Species : | Mouse |
Tag : | His |
Form : | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
Molecular Mass : | 23.5kDa |
Protein length : | Phe231~Ser425 |
Endotoxin : | <1.0EU per 1ug (determined by the LAL method) |
Purity : | > 95% |
Applications : | Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
Reconstitution : | Reconstitute in PBS or others |
Gene Name : | Smad3 MAD homolog 3 (Drosophila) [ Mus musculus ] |
Official Symbol : | Smad3 |
Synonyms : | SMAD3; MAD homolog 3 (Drosophila); mothers against decapentaplegic homolog 3; mad3; mMad3; Smad 3; SMAD family member 3; mothers against DPP homolog 3; Madh3; AU022421; |
Gene ID : | 17127 |
mRNA Refseq : | NM_016769 |
Protein Refseq : | NP_058049 |
UniProt ID : | Q8BUN5 |
Products Types
◆ Recombinant Protein | ||
SMAD3-2041H | Recombinant Human SMAD3 Protein, His (Fc)-Avi-tagged | +Inquiry |
SMAD3-5267R | Recombinant Rat SMAD3 Protein, His (Fc)-Avi-tagged | +Inquiry |
Smad3-5951M | Recombinant Mouse Smad3 Protein, Myc/DDK-tagged | +Inquiry |
SMAD3-4147R | Recombinant Rhesus Macaque SMAD3 Protein, His (Fc)-Avi-tagged | +Inquiry |
SMAD3-304H | Recombinant Human SMAD3 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
SMAD3-001MCL | Recombinant Mouse SMAD3 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewHigh expression level, good stability, suitable for high precision experiments.
Compared with other brands I have used, this brand is more stable and reliable.
Strong antibacterial activity, not easy to be contaminated.
Q&As (6)
Ask a questionThe expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.
interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.
In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.
expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.
The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.
SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.
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