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CAT

  • Official Full Name

    Chloramphenicol acetyltransferase

  • Overview

    Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme (EC 2.3.1.28) that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria. This enzyme covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, which prevents chloramphenicol from binding to ribosomes. A histidine residue, located in the C-terminal section of the enzyme, plays a central role in its catalytic mechanism. The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilised by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilisation is provided by an unusual interaction with a main-chain carbonyl oxygen.
  • Synonyms

    E. coli Chloramphenicol acetyltransferase; E. coli CAT; Escherichia coli Chloramphenicol acetyltransferase; Escherichia coli CAT; CAT;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Native Proteins
  • Protein Pre-coupled Magnetic Beads
  • Bell pepper
  • Bovine
  • Chicken
  • Human
  • Mouse
  • Rat
  • Staphylococcus
  • Zebrafish
  • Bovine Liver
  • E. coli or Yeast
  • E.coli
  • HEK293
  • HEK293T
  • Human Erythrocyte
  • In Vitro Cell Free System
  • Insect Cell
  • Mammalian Cell
  • Wheat Germ
  • GST
  • His
  • His (Fc)
  • Avi
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
  • Tag Free
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human CAT-2514H Active Recombinant Human CAT Protein, His-tagged, Animal Free, Beta-lactam Antibiotics Free E.coli His
Human CAT-167H Recombinant Human CAT Mammalian Cell His
Human CAT-176H Recombinant Human CAT Protein E.coli N/A
Human CAT-1646H Recombinant Human Catalase Human Erythrocyte N/A
Human CAT-0443H Recombinant Human CAT Protein, GST-Tagged Wheat Germ GST
Human CAT-2500H Recombinant Human CAT, His tagged Insect Cell His
Human CAT-515HCL Recombinant Human CAT cell lysate Insect Cell N/A
Human CAT-26409TH Native Human CAT N/A
Human CAT-5276H Native Human, Catalase Human Erythrocyte N/A
Human CAT-1609H Recombinant Human CAT Protein (Full Length), N-His tagged E.coli N-His Full Length
Human CAT-75H Native Human Catalase Human Erythrocyte N/A
Human CAT-4608H Recombinant Human CAT Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human CAT-2728H Recombinant Human CAT Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human CAT-630H Recombinant Human CAT Protein, His-tagged E.coli N-His Asp10-Asn507
Human CAT-21H Native Human Catalase Protein Human Erythrocyte N/A
Human CAT-2597H-B Recombinant Human CAT Protein Pre-coupled Magnetic Beads HEK293
Human CAT-654H Recombinant Human CAT Protein, His-tagged E.coli His Ala401~Glu523
Human CAT-629H Recombinant Human CAT Protein, His-tagged E.coli N-His Asp10-Asn507
Human CAT-631H Recombinant Human CAT Protein, His-tagged E.coli N-His Asp10-Asn507
Human CAT-2752HF Recombinant Full Length Human CAT Protein, GST-tagged In Vitro Cell Free System GST 527 amino acids
Human CAT-2597H Recombinant Human CAT Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human CAT-0865H Recombinant Human CAT Protein (Met1-Leu527), His tagged E.coli His Met1-Leu527
Human CAT-1600H Recombinant Human CAT Protein (Ala2-Leu527) E.coli Tag Free Ala2-Leu527
Mouse CAT-2765M Recombinant Mouse CAT Protein Mammalian Cell His
Mouse Cat-25M Recombinant Mouse Cat protein, His-tagged E.coli His
Mouse Cat-26M Recombinant Mouse Cat protein, His-tagged E.coli His
Mouse Cat-632M Recombinant Mouse Cat Protein, His-tagged E.coli N-His Ser2-Leu527
Mouse CAT-1252M Recombinant Mouse CAT Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Cat-658M Recombinant Mouse Cat Protein, His-tagged E.coli His Gly80~Asp239
Mouse CAT-1252M-B Recombinant Mouse CAT Protein Pre-coupled Magnetic Beads HEK293
Mouse Cat-2639M Recombinant Mouse Cat protein, His&Myc-tagged E.coli His&Myc 2-527aa
Rat CAT-1150R Recombinant Rat CAT Protein Mammalian Cell His
Rat CAT-808R Recombinant Rat CAT Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat CAT-808R-B Recombinant Rat CAT Protein Pre-coupled Magnetic Beads HEK293
Rat Cat-653R Recombinant Rat Cat Protein, His-tagged E.coli His Gly452~Thr658
Bell pepper CAT-08B Recombinant Bell pepper CAT protein, His-tagged E.coli His 1-492aa
Bovine CAT-101B Active Native Bovine CAT Bovine Liver N/A
Bovine CAT-1847B Active Native Bovine, Catalase Bovine Liver N/A
Bovine CAT-1187B Native Bovine Catalase Bovine Liver N/A
Staphylococcus CAT-1537S Recombinant Staphylococcus aureus (strain: TP4) CAT protein, His-tagged E. coli or Yeast His
Staphylococcus CAT-1568S Recombinant Staphylococcus aureus (strain: EP5) CAT protein, His-tagged E. coli or Yeast His
Zebrafish CAT-8553Z Recombinant Zebrafish CAT Mammalian Cell His
Chicken CAT-2878C Recombinant Chicken CAT Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • CAT Related Articles

CAT involved in several pathways and played different roles in them. We selected most pathways CAT participated on our site, such as Tryptophan metabolism, Glyoxylate and dicarboxylate metabolism, Carbon metabolism, which may be useful for your reference. Also, other proteins which involved in the same pathway with CAT were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Tryptophan metabolismHADHA;KYNU;GCDHB;AOC1;AANAT;HADHAA;ALDH2;TPH1;ALDH9A1B
Glyoxylate and dicarboxylate metabolismHYI;PCCB;GLULC;ACO2;AFMID;MUT;GRHPRA;MDH1;MDH1AA
Carbon metabolismSHMT1;PRPS2;GPT2L;ACSS2L;OGDH;FH;EHHADH;IDH3B;PKMA
FoxO signaling pathwayPRKAG3B;PDPK1;MAPK8;INSB;G6PCA.2;CCND2;PRKAG3;PCK2;FOXO3
PeroxisomeCRATB;ACOX3;ACSL3B;ACSL1B;ECH1;ACSL1;SOD1;HACL1;PEX11A
Amyotrophic lateral sclerosis (ALS)TOMM40;TNF;MAP3K5;BAX;NOS1;MAPK14;CASP12;MAPK12;GRIN2D

CAT has several biochemical functions, for example, NADP binding, aminoacylase activity, antioxidant activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CAT itself. We selected most functions CAT had, and list some proteins which have the same functions with CAT. You can find most of the proteins on our site.

Function Related Protein
NADP bindingFMO1;NOS3;HMGCRB;NOS1;NOX5;CRYM;POR;H6PD;G6PDX
aminoacylase activityDARS;CAT;ACY3;ACY1;ASPA
antioxidant activityFABP1;APOE;APOM;TP53INP1;PRDX5;FAM213AA;CAT;HP;PRDX2
catalase activityGPX7;CAT;CYGB
enzyme bindingAPOA1;TH;ATXN10;CA;HNRNPUL1;PLSCR1;UGT1A3;BRCA1;KAT8
heme bindingCYP2V1;TPO;CYP3C4;DGCR8;NGB;HBBE3;MPO;HBAE1;SRC
metal ion bindingZBTB2B;Ar;SCAPER;LHX8A;EXOG;GLIS2B;PDE9A;NME2;ADPRHL2
oxidoreductase activity, acting on peroxide as acceptorPARK7;CAT
protein homodimerization activityCPNE1;GSS;DARS2;CREB3;ACAT1;ASNS;CHMP1A;MTMR2;CHMP4C
receptor bindingH2-Q10;HAND2;LAMA2;DHRS4;FGF1A;AMH;FASLG;INSL4;SLC39A1

CAT has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CAT here. Most of them are supplied by our site. Hope this information will be useful for your research of CAT.

PTPN11; APP; APP; USP53; o68769_yerpe; p29991-pro_0000037946; KEAP1; proS; fcuA; leuB; 15-deoxy-delta(12,14; midostaurin; JUP; SMARCA4; ACTR3; GNAS

Butz, E; Peichl, L; et al. Cone Bipolar Cells in the Retina of the Microbat Carollia perspicillata. JOURNAL OF COMPARATIVE NEUROLOGY 523:963-981(2015).
Cong, W; Liu, GH; et al. Toxoplasma gondii infection in cancer patients: Prevalence, risk factors, genotypes and association with clinical diagnosis. CANCER LETTERS 359:307-313(2015).
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