tyms
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  • Official Full Name
  • thymidylate synthetase
  • Background
  • Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. 5,10-methylenetetrahydrofolate dUMP dihydrofolate dTMP By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product. This provides the sole de novo pathway for production of dTMP and is the only enzyme in folate metabolism in which the 5,10-methylenetetrahydrofolate is oxidised during one-carbon transfer. The enzyme is essential for regulating the balanced supply of the 4 DNA precursors in normal DNA replication: defects in the enzyme activity affecting the regulation process cause various biological and genetic abnormalities, such as thymineless death.[3] The enzyme is an important target for certain chemotherapeutic drugs. Thymidylate synthase is an enzyme of about 30 to 35 Kd in most species except in protozoan and plants where it exists as a bifunctional enzyme that includes a dihydrofolate reductase domain.[2] A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate). The sequence around the active site of this enzyme is conserved from phages to vertebrates. As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, the most notable one being raltitrexed (trade name Tomudex).
  • Synonyms
  • d TMP synthase; EC 2.1.1.45; HsT422; MGC88736; OTTHUMP00000162195; Thymidylate synthase; thymidylate synthetase; TMS; TS; TSase; TYMS; TYMS protein; Tyms thymidylate synthetase
Cat.#:TYMS-616HTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human TYMS Protein, His-tagged
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Cat.#:TYMS-30404THTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human TYMS, His-tagged
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Cat.#:TYMS-30405THTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human TYMS, His-tagged
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Cat.#:TYMS-3509HTag:GST
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human TYMS, GST-tagged
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Cat.#:TYMS-490HTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human Thymidylate Synthase, His tagged
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Cat.#:TYMS-684HTag:MYC/DDK
Source (Host):HEK293Species:Human
Product nameRecombinant Human TYMS protein, MYC/DDK-tagged
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Cat.#:TYMS-685HTag:His
Source (Host):Insect cellsSpecies:Human
Product nameRecombinant Human TYMS protein, His-tagged
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Cat.#:TYMS-17672MTag:His
Source (Host):Mammalian CellsSpecies:Mouse
Product nameRecombinant Mouse TYMS Protein
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Cat.#:TYMS-6381RTag:His
Source (Host):Mammalian CellsSpecies:Rat
Product nameRecombinant Rat TYMS Protein
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Cat.#:TYMS-9274ZTag:His
Source (Host):Mammalian CellsSpecies:Zebrafish
Product nameRecombinant Zebrafish TYMS
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Cat.#:TYMS-1868HCLTag:
Source (Host):Species:Human
Product nameRecombinant Human TYMS cell lysate
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Involved Pathway

TYMS involved in several pathways and played different roles in them. We selected most pathways TYMS participated on our site, such as Pyrimidine metabolism, One carbon pool by folate, Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with TYMS were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name
Pathway Related Protein
Pyrimidine metabolism
TXNRD3; POLR3K; ENTPD3; PRIM1; UPP1; CANT1B; POLR2D; POLR2H; NT5C2B; POLD4
One carbon pool by folate
TYMS; AMT; MTFMT; MTHFS; ALDH1L2; ALDH1L1; ATIC; MTHFD2; FTCD; GART
Metabolic pathways
G6PC; DCTPP1; ST6GAL2; CEPT1B; UGT5G1; ATP5A1; SPTLC2A; CYP27A1; IDS; MGAT2

Protein Function

TYMS has several biochemical functions, for example, cofactor binding, drug binding, folic acid binding. Some of the functions are cooperated with other proteins, some of the functions could acted by TYMS itself. We selected most functions TYMS had, and list some proteins which have the same functions with TYMS. You can find most of the proteins on our site.
Function
Related Protein
Function cofactor binding
Related Protein ASNS; HACL1; DAO; PGAM2; DDO; C3; PHYH; WWOX; ACLYA; PAH
Function drug binding
Related Protein CHRNA2; SRP54B; MT2A; PIGC; RARB; PDE10A; CHRM2; FOLR1; CYP4B1; ADH1
Function folic acid binding
Related Protein MTHFS; TYMS; GNMT; FOLR1; DAAM2; FOLR2; SLC19A1; FTCD; SLC46A1; DMGDH
Function mRNA binding
Related Protein PARK7; CSTF2; IGF2BP2A; RPL13A; TSN; RPS5; CSTF2T; CELF3; NUDT16; DOM3Z
Function nucleotide binding
Related Protein HINT3; TUBA4L; UBE2NB; GKUP; APOA1BP; NLRP4C; RBM19; NIM1; GBP3; ABCA5
Function protein homodimerization activity
Related Protein ALDH1A3; MAP3K10; MID2; CAT; UGT1A2; HNF4A; BCL11A; TCF3B; MGST1; ANO6
Function thymidylate synthase activity
Related Protein TYMS

Interacting Protein

TYMS has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with TYMS here. Most of them are supplied by our site. Hope this information will be useful for your research of TYMS.
VCAM1; SH3GL2; Tuba3a

TYMS Related Articles

Rupasree, Y; Naushad, SM; et al. Epigenetic modulation of RFC1, MHC2TA and HLA-DR in systemic lupus erythematosus: Association with serological markers and six functional polymorphisms of one-carbon metabolic pathway. GENE 536:45-52(2014).
Etienne-Grimaldi, MC; Bennouna, J; et al. Multifactorial pharmacogenetic analysis in colorectal cancer patients receiving 5-fluorouracil-based therapy together with cetuximab-irinotecan. BRITISH JOURNAL OF CLINICAL PHARMACOLOGY 73:776-785(2012).