| Description : |
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
35.5 kDa |
| AA Sequence : |
MPVAGSELPRRPLPPAAQERDAEPR PPHGELQYLGQIQHILRCGVRKDDR TGTGTLSVFGMQARYSLRDEFPLLT TKRVFWKGVLEELLWFIKGSTNAKE LSSKGVKIWDANGSRDFLDSLGFST REEGDLGPVYGFQWRHFGAEYRDME SDYSGQGVDQLQRVIDTIKTNPDDR RIIMCAWNPRDLPLMALPPCHALCQ FYVVNSELSCQLYQRSGDMGLGVPF NIASYALLTYMIAHITGLKPGDFIH TLGDAHIYLNHIEPLKIQLQREPRP FPKLRILRKVEKIDDFKAEDFQIEG YNPHPTIKMEMAVTRTRPLEQKLIS EEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
