Recombinant Human TYMS protein, His-tagged

• Cat.No.: TYMS-685H
• Product Overview: Recombinant Human TYMS fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
• Form: 50mM Tris-HCl pH8.0, 150mM NaCl, 10% glycerol.
• Molecular Mass: 36 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: TYMS thymidylate synthetase [ Homo sapiens ]
• Official Symbol: TYMS
• Synonyms: TYMS; thymidylate synthetase; TS; thymidylate synthase; HsT422; TMS; Tsase; TSase; HST422; MGC88736
• Gene ID: 7298
• mRNA Refseq: NM_001071
• Protein Refseq: NP_001062
• MIM: 188350
• UniProt ID: P04818
• Chromosome Location: 18p11.31-p11.21
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; G1/S Transition, organism-specific biosystem; G1/S-Specific Transcription, organism-specific biosystem
• Function: cofactor binding; folic acid binding; methyltransferase activity; nucleotide binding; thymidylate synthase activity; thymidylate synthase activity; transferase activity