Recombinant Human IRAK1 Protein (R194-S712), GST tagged

• Cat.No.: IRAK1-1171H
• Product Overview: Recombinant Human GST-TEV-GG-IRAK1(R194-S712 end) Protein was expressed in Insect cell.

Specification

• Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
• Source: Insect cell
• Species: Human
• Tag: GST
• Form: Liquid
• Protein length: R194-S712
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: IRAK1 interleukin-1 receptor-associated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: IRAK1
• Synonyms: IRAK1; interleukin-1 receptor-associated kinase 1; IRAK; pelle; IRAK-1; Pelle homolog
• Gene ID: 3654
• mRNA Refseq: NM_001025242
• Protein Refseq: NP_001020413
• MIM: 300283
• UniProt ID: P51617

Reviews

04/01/2021

The IRAK1 protein stands out for its exceptional quality, making it an excellent choice to meet my experimental requirements.

04/21/2020

The IRAK1 protein's compatibility with various experimental techniques adds to its versatility.

05/12/2017

Its stability and compatibility make it an excellent tool for visualizing and characterizing protein structures at high resolution.

Q&As

Are there any ongoing clinical trials targeting IRAK1 for cancer treatment?

Yes, there are several clinical trials exploring the inhibition of IRAK1 as a therapeutic strategy for various types of cancer.

How is IRAK1 protein implicated in autoimmune diseases?

IRAK1 is involved in the signaling pathways that can lead to the development of autoimmune diseases by regulating the immune system's response to self-antigens.

How does the expression of IRAK1 relate to inflammatory diseases?

Elevated levels of IRAK1 expression have been observed in inflammatory diseases, suggesting its involvement in the dysregulation of inflammatory responses.

In what ways can IRAK1 be a target for drug development in inflammatory conditions?

Inhibiting IRAK1 activity is being investigated as a potential therapeutic approach for managing inflammatory conditions, offering a target for drug development.

What clinical significance does IRAK1 hold in cancer research?

IRAK1 has been identified as a potential target for cancer therapy, as it plays a role in promoting tumor growth and evasion of the immune system.