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RFC5

Official Full Name

replication factor C (activator 1) 5, 36.5kDa
Overview

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.
Synonyms

RFC5; replication factor C (activator 1) 5, 36.5kDa; replication factor C (activator 1) 5 (36.5kD); replication factor C subunit 5; RFC36; 36 kDa subunit; A1 36; A1 36 kDa subunit; A136; Activator 1 36 kDa subunit; Activator 1; Activator 1 subunit 5; Activator 136 kDa subunit; MGC1155; Replication factor C 5 isoform 1; Replication Factor C; Replication Factor C36 kDa subunit; Replication factor c5; RFC 36.5 kD subunit; RFC 5; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; replication factor C, 36-kDa subunit;

Type

Cell & Tissue Lysates
Recombinant Proteins
Protein Pre-coupled Magnetic Beads

Species

Human
Mouse
Rhesus Macaque
Zebrafish

Source

E.coli
HEK293
In Vitro Cell Free System
Mammalian Cell
Wheat Germ

Tag

GST
His
His (Fc)
Avi
His|T7
N/A

Species	Cat.#	Product name	Source (Host)	Tag	Protein Length	Price
Human	RFC5-2409HCL	Recombinant Human RFC5 293 Cell Lysate	HEK293	N/A		Inquiry
Human	RFC5-01H	Recombinant Human RFC5 Protein, GST-tagged	Wheat Germ	GST		Inquiry
Human	RFC5-1699H	Recombinant Human RFC5 protein, His & T7-tagged	E.coli	His/T7	Met1~Ile219 (Accession # P40937)	Inquiry
Human	RFC5-3337H	Recombinant Human RFC5 protein, His-tagged	E.coli	His	219-341 aa	$319 / 25ug
Human	RFC5-6870HF	Recombinant Full Length Human RFC5 Protein, GST-tagged	In Vitro Cell Free System	GST	340 amino acids	Inquiry
Mouse	Rfc5-1700M	Recombinant Mouse Rfc5 protein, His & T7-tagged	E.coli	His/T7	Thr63~Ala339 (Accession # Q9D0F6)	Inquiry
Rhesus Macaque	RFC5-3859R	Recombinant Rhesus monkey RFC5 Protein, His-tagged	Mammalian Cell	His		Inquiry
Rhesus Macaque	RFC5-3676R-B	Recombinant Rhesus Macaque RFC5 Protein Pre-coupled Magnetic Beads	HEK293			Inquiry
Rhesus Macaque	RFC5-3676R	Recombinant Rhesus Macaque RFC5 Protein, His (Fc)-Avi-tagged	HEK293	His (Fc)-Avi		Inquiry
Zebrafish	RFC5-1149Z	Recombinant Zebrafish RFC5	Mammalian Cell	His		Inquiry

Involved Pathway
Protein Function
Interacting Protein
RFC5 Related Articles

RFC5 involved in several pathways and played different roles in them. We selected most pathways RFC5 participated on our site, such as DNA replication, Nucleotide excision repair, Mismatch repair, which may be useful for your reference. Also, other proteins which involved in the same pathway with RFC5 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name	Pathway Related Protein
DNA replication	PCNA;rnhA;GMNN;POLE3;RFC5;GINS3;RNASEH2A;POLE4;POLE
Nucleotide excision repair	COPS6;POLD4;CHD1L;ZNF830;RFC3;RAD23B;GTF2H2C_2;POLE;MNAT1
Mismatch repair	MSH3;POLD2;POLD4;MLH1;RPA1;PMS2;MSH2;PCNA;RFC2

RFC5 has several biochemical functions, for example, ATP binding, contributes_to DNA clamp loader activity, enzyme binding. Some of the functions are cooperated with other proteins, some of the functions could acted by RFC5 itself. We selected most functions RFC5 had, and list some proteins which have the same functions with RFC5. You can find most of the proteins on our site.

Function	Related Protein
ATP binding	MOV10B.1;CHUK;ACTR2B;SGK2;PMELA;CAMK2B1;DDX21;ATP10A;DDX5
contributes_to DNA clamp loader activity	RFC2;RFC5;DSCC1;RFC4
enzyme binding	UGT1A1;HIF1A;GNB2L1;ECM1;BRCA1;CYP1A1;PTPLAD1;DNMT3L;UBE4B
protein binding	CRY2;PRKD1;KIR2DL4;FAM162A;NUP62CL;Anks6;WBP1;SLC38A2;PRDM10

RFC5 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with RFC5 here. Most of them are supplied by our site. Hope this information will be useful for your research of RFC5.

RFC4; TRIM38; XIAP; NAB2

Liu, D; Zhang, XX; et al. Sine oculis homeobox homolog 1 promotes DNA replication and cell proliferation in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY 45:1232-1240(2014).

Liu, SM; Chen, WP; et al. Distinguishing between cancer cell differentiation and resistance induced by all-trans retinoic acid using transcriptional profiles and functional pathway analysis. SCIENTIFIC REPORTS 4:-(2014).