Recombinant Full Length Human RFC5 Protein, GST-tagged

• Cat.No.: RFC5-6870HF
• Product Overview: Human RFC5 full-length ORF ( AAH13961, 1 a.a. - 340 a.a.) recombinant protein with GST-tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants.
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 63.14 kDa
• Protein Length: 340 amino acids
• AA Sequence: METSALKQQEQPAATKIRNLPWVEK YRPQTLNDLISHQDILSTIQKFINE DRLPHLLLYGPPGTGKTSTILACAK QLYKDKEFGSMVLELNASDDRGIDI IRGPILSFASTRTIFKKGFKLVILD EADAMTQDAQNALRRVIEKFTENTR FCLICNYLSKIIPALQSRCTRFRFG PLTPELMAPRLEHVVEEEKVDISED GMKALVTLSSGDMRRALNILQSTNM AFGKVTEETVYTCTGHPLKSDIANI LDWMLNQDFTTAYRNITELKTLKGL ALHDILTEIHLFVHRVDFPSSVRIH LLTKMADIEYRLSVGTNEKIQLSSL IAAFQVTRDLIVAEA
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: RFC5 replication factor C subunit 5 [ Homo sapiens (human) ]
• Official Symbol: RFC5
• Synonyms: RFC5; replication factor C subunit 5; RFC36; replication factor C subunit 5; A1 36 kDa subunit; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; replication factor C (activator 1) 5, 36.5kDa; replication factor C, 36-kDa subunit
• Gene ID: 5985
• mRNA Refseq: NM_181578
• Protein Refseq: NP_853556
• MIM: 600407
• UniProt ID: P40937