Active Recombinant Bat coronavirus Spike Protein (K978P, V979P), His&Twin-Strep-tagged
Cat.No. : | Spike-4795B |
Product Overview : | Recombinant Bat coronavirus Protein (K978P, V979P)(Met 1 - Gln 1200), fused with His and Twin-Strep tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Bat coronavirus |
Tag : | His&Twin-Strep |
Form : | Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.1-4 ng/mL.Immobilized Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-2 ng/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag.The protein has a calculated MW of 141.2 kDa. The protein migrates as 170-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Met 1 - Gln 1200 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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