"RIPK3" Related Products

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Recombinant Human RIPK3 293 Cell Lysate

Cat.No.: RIPK3-2332HCL
Description: Antigen standard for receptor-interacting serine-threonine kinase 3 (RIPK3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RIPK3 receptor-interacting serine-threonine kinase 3 [ Homo sapiens ]
Official Symbol: RIPK3
Synonyms: RIPK3; receptor-interacting serine-threonine kinase 3; receptor-interacting serine/threonine-protein kinase 3; RIP3; RIP-3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3;
Gene ID: 11035
mRNA Refseq: NM_006871
Protein Refseq: NP_006862
MIM: 605817
UniProt ID: Q9Y572
Chromosome Location: 14q12
Pathway: Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; RIP-mediated NFkB activation via DAI, organism-specific biosystem;
Function: ATP binding; NF-kappaB-inducing kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transcription coactivator activity;

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