Recombinant Human PKN2 293 Cell Lysate

• Cat.No.: PKN2-3150HCL

Specification

• Description: Antigen standard for protein kinase N2 (PKN2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PKN2 protein kinase N2 [ Homo sapiens ]
• Official Symbol: PKN2
• Synonyms: PKN2; protein kinase N2; PRKCL2, protein kinase C like 2; serine/threonine-protein kinase N2; cardiolipin activated protein kinase Pak2; Pak 2; PRK2; PKN gamma; protein kinase C-like 2; protein-kinase C-related kinase 2; cardiolipin-activated protein kinase Pak2; PAK2; Pak-2; PRKCL2; PRO2042; MGC71074; MGC150606
• Gene ID: 5586
• mRNA Refseq: NM_006256
• Protein Refseq: NP_006247
• MIM: 602549
• UniProt ID: Q16513
• Chromosome Location: 1p22
• Pathway: EGFR1 Signaling Pathway, organism-specific biosystem; RhoA signaling pathway, organism-specific biosystem; Salmonella infection, organism-specific biosystem; Salmonella infection, conserved biosystem
• Function: ATP binding; histone deacetylase binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase activity; protein serine/threonine kinase activity