Active Recombinant Human PHKG1, GST-tagged

• Cat.No.: PHKG1-1074H
• Product Overview: Recombinant full-length human PHKG1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 29 nmol/min/mg
• Molecular Mass: ~70 kDa
• Protein length: Full Length
• Purity: >75%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70°C from date of shipment.
• Storage: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PHKG1 phosphorylase kinase, gamma 1 (muscle) [ Homo sapiens(human) ]
• Official Symbol: PHKG1
• Synonyms: PHKG1; phosphorylase kinase, gamma 1 (muscle); phosphorylase kinase, gamma 1 (muscle); phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform; phosphorylase kinase gamma; phosphorylase kinase subunit gamma-1; EC 2.7.11.19
• Gene ID: 5260
• mRNA Refseq: NM_006213
• Protein Refseq: NP_006204
• MIM: 172470
• UniProt ID: Q16816
• Chromosome Location: 7p11.2
• Pathway: Calcium signaling pathway; Insulin signaling pathway; Metabolism of carbohydrates
• Function: ATP binding; calmodulin binding; enzyme binding; phosphorylase kinase activity