Recombinant Mouse Eph Receptor B1, GST-tagged, Active

• Cat.No.: Ephb1-303M
• Product Overview: Recombinant mouse Ephb1 (591-end) was expressed by baculovirus inSf9 insect cellsusing an N-terminal GST tag. MW=62kDa.

Specification

• Cat. No.: Ephb1-303M
• Description: EPHB1 is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration. In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis.
• Source: Sf9 insect cellsusing baculovirus.
• Sequence: 591-end.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: Ephb1 Eph receptor B1 [ Mus musculus ]
• Synonyms: Ephb1; Eph receptor B1; Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
• Gene ID: 270190
• mRNA Refseq: NM_001168296
• Protein Refseq: NP_001161768
• UniProt ID: Q8CBF3
• Chromosome Location: 9F1
• Pathway: Axon guidance
• Function: ATP binding; ephrin receptor activity; nucleotide binding; protein binding; receptor activity; transferase activity; protein kinase activity; protein tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; transmembrane-ephrin receptor activity