|Cat. No. :
|Product Overview :
||Recombinant mouse Ephb1 (591-end) was expressed by baculovirus inSf9 insect cellsusing an N-terminal GST tag. MW=62kDa.
||EPHB1 is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration. In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis.
||Sf9 insect cellsusing baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability :
||Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.