Recombinant Human GCK, His-tagged

• Cat.No.: GCK-29572TH
• Product Overview: Human Recombinant Full length IGLKis a single, non-glycosylated, polypeptide chain with an N terminal His tag.485 amino acids,54 kDa.

Specification

• Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris, pH 8
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ.
• Sequence Similarities: Belongs to the hexokinase family.

Gene Information

• Gene Name: GCK glucokinase (hexokinase 4) [ Homo sapiens ]
• Official Symbol: GCK
• Synonyms: GCK; glucokinase (hexokinase 4); maturity onset diabetes of the young 2 , MODY2; glucokinase; HK4
• Gene ID: 2645
• mRNA Refseq: NM_000162
• Protein Refseq: NP_000153
• MIM: 138079
• Uniprot ID: P35557
• Chromosome Location: 7p15.3-p15.1
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Butirosin and neomycin biosynthesis, organism-specific biosystem; Butirosin and neomycin biosynthesis, conserved biosystem; Developmental Biology, organism-specific biosystem
• Function: ATP binding; glucokinase activity; glucose binding; kinase activity; nucleotide binding