NIH/3T3 Whole Cell Lysate
| Cat.No. : | Mouse Embryo-152M |
- Specification
- Gene Information
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| Species : | Mouse |
| Form : | The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Concentration : | 2.0 mg/ml |
| Cell line : | NIH/3T3 (Embryo) |
| Tissue Type : | Mouse Embryo |
| Growth media : | DMEM & 10% FBS (Fetal Bovine Serum) |
| Preparation method : | 3T3 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. |
| Expiration Date : | One year from date of receipt |
| Size : | 0.1 mg, 0.5 mg |
| Storage Instruction : | This whole cell lysate is stable for three months at -20°C |
| Applications : | This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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