Recombinant Cynomolgus FCGRT/B2M protein, His/Strep II/Avi-tagged, Biotinylated

• Cat.No.: FCGRT & B2M-1535C
• Product Overview: Recombinant Cynomolgus FCGRT(Ala 24 - Ser 297)/B2M(Ile 21 - Met 119), subunit FCGRT / FcRN carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His/Strep II/Avi
• Predicted N Terminal: Ala 24 (FcRN) & Ile 21 (B2M)
• Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4) with Trehalose as protectant.
• Molecular Mass: Has a calculated MW of 34.1 kDa (FcRN), 13.1 kDa (B2M) . The protein migrates as 40 kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Protein length: FCGRT(Ala 24 - Ser 297)/B2M(Ile 21 - Met 119)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Gene Information

• Gene Name: FCGRT Fc fragment of IgG receptor and transporter [ Macaca fascicularis ]
• Official Symbol: FCGRT
• Synonyms: Fc fragment of IgG, receptor, transporter, alpha; IgG Fc fragment receptor transporter alpha chain; Neonatal Fc receptor
• Gene ID: 102128913
• mRNA Refseq: NM_001284551
• Protein Refseq: NP_001271480
• UniProt ID: Q8SPV9
• Chromosome Location: chromosome: 19