Recombinant Human ACSS2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ACSS2-4280H
• Product Overview: ACSS2 MS Standard C13 and N15-labeled recombinant protein (NP_061147) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 78.6 kDa
• AA Sequence: MGLPEERVRSGSGSRGQEEAGAGGR ARSWSPPPEVSRSAHVPSLQRYREL HRRSVEEPREFWGDIAKEFYWKTPC PGPFLRYNFDVTKGKIFIEWMKGAT TNICYNVLDRNVHEKKLGDKVAFYW EGNEPGETTQITYHQLLVQVCQFSN VLRKQGIQKGDRVAIYMPMIPELVV AMLACARIGALHSIVFAGFSSESLC ERILDSSCSLLITTDAFYRGEKLVN LKELADEALQKCQEKGFPVRCCIVV KHLGRAELGMGDSTSQSPPIKRSCP DVQISWNQGIDLWWHELMQEAGDEC EPEWCDAEDPLFILYTSGSTGKPKG VVHTVGGYMLYVATTFKYVFDFHAE DVFWCTADIGWITGHSYVTYGPLAN GATSVLFEGIPTYPDVNRLWSIVDK YKVTKFYTAPTAIRLLMKFGDEPVT KHSRASLQVLGTVGEPINPEAWLWY HRVVGAQRCPIVDTFWQTETGGHML TPLPGATPMKPGSATFPFFGVAPAI LNESGEELEGEAEGYLVFKQPWPGI MRTVYGNHERFETTYFKKFPGYYVT GDGCQRDQDGYYWITGRIDDMLNVS GHLLSTAEVESALVEHEAVAEAAVV GHPHPVKGECLYCFFTLCDGHTFSP KLTEELKKQIREKIGPIATPDYIQN APGLPKTRSGKIMRRVLRKIAQNDH DLGDMSTVADPSVISHLFSHRCLTI QTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ACSS2 acyl-CoA synthetase short-chain family member 2 [ Homo sapiens (human) ]
• Official Symbol: ACSS2
• Synonyms: ACSS2; acyl-CoA synthetase short-chain family member 2; ACAS2, acetyl Coenzyme A synthetase 2 (ADP forming); acetyl-coenzyme A synthetase, cytoplasmic; AceCS; ACS; ACSA; dJ1161H23.1; acetate thiokinase; acetate-CoA ligase; acyl-activating enzyme; cytoplasmic acetyl-coenzyme A synthetase; acetyl-Coenzyme A synthetase 2 (ADP forming); ACAS2; ACECS; DKFZp762G026
• Gene ID: 55902
• mRNA Refseq: NM_018677
• Protein Refseq: NP_061147
• MIM: 605832
• UniProt ID: Q9NR19