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Active Recombinant Human FGL1 Protein, HIgG1 Fc-Avi-tagged, Biotinylated

Cat.No. : FGL1-119HB
Product Overview : Biotinylated Recombinant Human FGL1 Protein (Leu23-Ile312) is produced by HEK293 Cells expression system. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
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Description : Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.
Source : HEK293
Species : Human
Tag : HIgG1 Fc-Avi
Predicted N Terminal : Leu 23
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, 10% trehalose.
Bio-activity : Immobilized Human LAG-3, Mouse IgG2a Fc tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human FGL1, Fc,Avitag with a linear range of 0.156-1.25 μg/mL.
Molecular Mass : The protein has a calculated MW of 62.3 kDa. The protein migrates as 65 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Protein length : Leu23-Ile312
Endotoxin : < 1.0 EU per μg by the LAL method.
Purity : > 95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : FGL1 fibrinogen-like 1 [ Homo sapiens ]
Official Symbol : FGL1
Synonyms : FGL1; fibrinogen-like 1; HFREP 1; hepassocin; HFREP1; HP-041; LFIRE1; LFIRE-1; MGC12455;
Gene ID : 2267
mRNA Refseq : NM_004467
Protein Refseq : NP_004458
MIM : 605776
UniProt ID : Q08830

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the challenges in the study of FGL1 recombinant protein? 09/28/2022

There are still some challenges in the study of FGL1 recombinant protein, such as the need to better understand its structure and function relationship, and develop more effective drugs or treatments. In addition, due to the complexity and individual differences of tumor diseases, the therapeutic strategy against FGL1 recombinant protein also needs to be adjusted and optimized according to individual conditions.

How can animal models be used to study the function and disease mechanism of FGL1 recombinant protein? 07/25/2022

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role and disease mechanism of FGL1 recombinant protein in vivo. These animal models can be used to study the effects of FGL1 recombinant protein on tumor growth and metastasis, among other things.

Which pathways are available to study the function of FGL1 recombinant proteins? 11/03/2021

The function of FGL1 recombinant protein can be studied by gene knockout, transgenesis, protein interaction and other techniques.

How does FGL1 recombinant protein interact with other proteins? 09/30/2021

FGL1 recombinant protein can interact with other proteins, such as cell surface receptors such as integrins, and participate in cell adhesion and migration processes.

What were the steps used to prepare the recombinant FGL1 protein in the experiment? 11/28/2020

In the experiment, the FGL1 gene can be introduced into the expression vector by gene cloning technology, and then the FGL1 recombinant protein can be expressed in Escherichia coli or eukaryotic cells.

How to detect the expression level of FGL1 recombinant protein? 04/27/2020

The expression level of FGL1 recombinant protein can be detected by immunohistochemistry, immunofluorescence staining, western blotting, etc.

Customer Reviews (3)

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Reviews
08/06/2023

    FGL1 exhibits excellent stability and reproducibility under specific experimental conditions.

    10/14/2021

      This protein has good stability and is suitable for long-term storage and use.

      07/21/2020

        FGL1 protein has a long shelf life, which facilitates the continuity of subsequent experiments.

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