Recombinant Human HLA-A/B2M & YLEPGPVTA protein, His-Avi-tagged, Biotinylated

• Cat.No.: HLA-A/B2M & YLEPGPVTA-769HB
• Product Overview: Biotinylated Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is expressed from mammalian with a His and avi tag at the C-terminal. It contains Gly 25-Thr 305 (HLA-A*0201),Ile21-Met119 (B2M) and YLEPGPVTA peptide.

Specification

• Description: Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLAA*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein has a calculated MW of 50.4kDa. The protein migrates to 52-58kDa based on Bis-Tris PAGE.
• Protein length: Gly 25-Thr 305 (HLA-A*0201),Ile21-Met119 (B2M) and YLEPGPVTA peptide
• Endotoxin: Less than 0.01EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A & B2M beta-2-microglobulin [Homo sapiens (human)]
• Official Symbol: HLA-A & B2M
• Synonyms: HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M,Melanocytes lineage-specific antigen GP100, P100
• Gene ID: 3105 & 567
• mRNA Refseq: NM_001242758.1 & NM_004048.4
• Protein Refseq: NP_001229687.1 & NP_004039.1
• MIM: 142800 & 109700
• UniProt ID: P30443 & P61769