Recombinant Human IL12B & IL23A protein(Arg20-Pro189&Ile23-Ser328), His-Avi-tagged, Biotinylated

• Cat.No.: IL12B & IL23A-1261H
• Product Overview: Biotinylated Recombinant Human IL12B & IL23A protein(Arg20-Pro189&Ile23-Ser328)(Q9NPF7&P29460) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Arg20-Pro189&Ile23-Ser328
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL12B interleukin 12B [ Homo sapiens (human) ]
• Official Symbol: IL12B & IL23A
• Synonyms: CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B; IL23A
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460